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Date Published
Mouse TNF-alpha ELISA MAX™ Standard
(1)
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BioLegend
October 31, 2014
Detect TNF-a production in LPS activated macrophage culture. It is an easy way to set up this traditional measure system.
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Evaluating CD62E expression by IF
(1)
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October 31, 2014
R and D systems mouse anti-human CD62E was tested on 293T human cells ectopically expressing human CD62E by immunoflourescence. Fixation and...
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Quickly assess cell death- Caspase 3/7 activation
(1)
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Promega
October 30, 2014
An easy to way to measure cell death in a plate- based assay. This kit allows the scientist to rapidly measure the levels of caspase-3/7 ...
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Phospho-ERK1/2 detection in HL-1 via Western blotting
(1)
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Cell Signaling Technology
October 30, 2014
Phospho-ERK1/2 protein expression was characterized via western blot in HL-1 cells
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Good enzyme, some optimization likely required
(1)
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New England Biolabs
October 29, 2014
A loop-mediated isothermal amplification (LAMP) study was conducted to detect the presence of pathogen DNA in food samples. Bst 2.0 from NEB...
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Rabbit anti-Hsp60 Ab for IF
(1)
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GeneTex
October 29, 2014
Immunofluorescence in MEFs staining for Hsp60. Hsp60 was chosen as a mitochondrial marker to examine the mitochondrial network in MEFs.
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Mouse IFN-gamma ELISA MAX™ Standard
(1)
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BioLegend
October 28, 2014
To measure the IFN-gamma production in conA activated T cell culture. To set up a measurement system for T cell activation.
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FGF2 protein expression via western blotting
(1)
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MilliporeSigma
October 27, 2014
FGF2 protein expression was characterized via western blot using the Anti-FGF-2/bFGF Antibody (1:500) in murine HL-1 cells.
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Great product for quick and easy miRNA extraction
(1)
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Thermo Fisher Scientific
October 24, 2014
The goal of the experiment was to examine microRNA and gene expression changes in the hypothalami of rats at Postnatal day 15, P30, P45, and...
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Excellent mouse IL-7 cytokine
(1)
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BioLegend
October 23, 2014
Basically, we are culturing one mouse B cell line which needs IL-7 for survival and proliferation. At the beginning, we used 10ng/ml, and ...
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Sigma FLAG Antibody
(1)
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October 22, 2014
We used this antibody for Western blotting of the FLAG-tagged proteins expressed in mammalian cell lines. Works well if stored properly. ...
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Highly recommend LA Taq Polymerase
(1)
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October 21, 2014
This taq is optimum to expand any gene from 1kb to 15kb. It works specifically great for the ones bigger than 2kb. The PCR product can also ...
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Western blotting: human CXCR4 (Fusin) MAb (Clone 12G5)
(1)
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R&D Systems, a Bio-Techne Brand
October 20, 2014
Western blotting was performed using anti-human CXCR4 (Fusin) antibody in humen endothelial cells
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Workhorse
(1)
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October 17, 2014
Our lab studies a specific subset of B cells. Our research focuses on determining the overall function of these B cells in both human and ...
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Beta-Actin protein expression analysis via Western blotting
(1)
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Santa Cruz Biotechnology, Inc.
October 16, 2014
Beta-Actin protein expression was characterized via Western blot using the anti-ß-Actin (1:10000) from Santa Cruz.
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AquaLab equipment works well
(1)
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October 15, 2014
We measure water activity for a number of foods. The AquaLab Water Activity Meter is very efficient at conducting these measurements and is ...
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SF2/ASF protein expression analyzes via Western blot
(1)
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Thermo Fisher Scientific
October 14, 2014
SF2/ASF protein expression was characterized via western blot using the SF2/ASF Antibody (dilution 1:1000).
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KAT6A antibody evaluated for Western blot and pull down experiment
(1)
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October 13, 2014
In my experiment we study protein-protein interaction between KAT6a and a KAT6a interacting protein. The antibody gave very nice pull-down ...
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Simple Diagnostic Test for STEC
(1)
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October 10, 2014
Our lab is in the pathogen detection field, we tested the The Verotoxin-producing Escherichia coli Detection Kit from SA Scientific to test ...
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Great primers at good prices
(1)
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October 09, 2014
I have had a great experience ordering primers from IDT. They have always given me high-quality primers, shipped very quickly, and their ...
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Thermo Scientific Precision Digital Water Bath, with Lab Armor Beads
(1)
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October 08, 2014
Using cell culture, our lab assesses the viability of mouse cortical cells through several assays, including radioactive uptake. Since our ...
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Solid workhorse machine
(1)
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Thermo Fisher Scientific
October 07, 2014
We wanted to perform qPCR testing to determine if treatment of a G-protein-coupled receptor with an agonist would affect the transcription ...
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Excellent antibody for immunofluoresence detection of YAP/TAZ in mouse cells
(1)
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Cell Signaling Technology
October 02, 2014
The goal of the study was to detect YAP/TAZ localization in mouse embryonic fibroblasts by immunofluorescence
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Very good antibody for detection of SHP2 by immunofluoresence
(1)
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October 01, 2014
The goal of this experiment was to determine the cellular localization of SHP2 in mouse embryonic fibroblasts (MEFs)
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BACH2 copy number analysis using Life Technologies TaqMan Assays
(1)
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September 30, 2014
I am interested in copy number variations of particular genes in B-cell lymphoma. I have used the Life Technologies Copy Number Assays to ...
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PE/Dazzle™ 594 anti-human CD19 Antibody
(1)
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BioLegend
September 26, 2014
Cells Depot™ preserved human PBMCs was stained with PE/Dazzle™ 594 anti-human CD19 Antibody diluted in flow cytometry buffer (PBS + 2% CS). ...
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Use of Extan PMP22 Kit by Centogene
(1)
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September 19, 2014
Extan Kit for PMP22 by Centogene was used to identify point mutations responsible for CMT1A / CMT1E. The instructions in the kit for the ...
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Quantifying the Terminal Complement Complex (C5b-9) in Plasma
(1)
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September 08, 2014
Activation of any of the three complement pathways (classical, alternative, or lectin) results in the formation of the terminal complement ...
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PPi Release Assay Works Well
(1)
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September 08, 2014
I used the Lonza PPi release assay to measure the activity of a pyrophosphorylase. I also compared the activity to a suspect ...
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Are your T cells activated?
(1)
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BioLegend
September 05, 2014
The stimulation of T cells is carried out by different methods. In order to check if your stimulation worked or not, there are many factors ...
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Detecting TGFß1 in Human Sarcoma
(1)
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Santa Cruz Biotechnology, Inc.
September 05, 2014
The TGF family of proteins are implicated in many tumor processes, such as development, growth, migration, and metastasis. A good antibody ...
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Muscle Actin Identification by IHC
(1)
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September 05, 2014
We use this antibody for some tumor types classifications. It can recognizes specific Actin present in skeletal muscle, not in smooth muscle...
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Western Blotting - CLK2 Antibody in Human Endothelial Cells
(1)
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Aviva Systems Biology
September 05, 2014
Clk2 protein expression was characterized via western blot using the CLK2 Antibody (1:1000).
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Determination of the IgG Subclass of a Human Autoantibody
(1)
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Abcam
September 02, 2014
Our laboratory is interested in an autoantibody that we identified several years ago in cancer patients. The presence of this autoantibody ...
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Great anti-FLAG Monoclonal Antibody
(1)
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September 02, 2014
A FLAG tagged construct was overexpressed in HEK293 cells and WB was utilized to track the expression level of the protein.
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Norgen Urine Total RNA Purification Kit
(1)
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Norgen Biotek Corporation
September 02, 2014
I am interested in the expression of genes in the urine of transplant, in order to identify biomarkers for a disease called graft versus ...
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DNA and Protein Visualization
(1)
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ProteinSimple, a Bio-Techne Brand
September 01, 2014
This equipment was installed as shared facility 3 years back at Ohio Sate University in building named Tzagournis. Our lab work involves ...
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PCR Purification via MinElute PCR Purification Kit
(1)
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September 01, 2014
We study the various mechanisms of gene silencing responsible for disease resistance in poultry. We specifically study various target genes ...
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Kit for isolation of DNA, total RNA and protein
(1)
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August 21, 2014
This kit was used to collect DNA, RNA, and protein for PCR, qPCR, and Western blotting for a circadian rhythms experiment. We thought that ...
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Use of Tendem Dye for Multicolor Flow Cytometry
(1)
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BioLegend
August 15, 2014
The aim of my study was to investigate CD4+ T cell proliferation. I harvest mouse splenocytes and use following antibodies from Biolegend ...
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Great Transfer Apparatus
(1)
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Bio-Rad
August 14, 2014
I use this apparatus to transfer proteins from SDS-PAGE gels to PVDF membranes for immunoblotting.
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Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb antibody (for Western Blot) from Cell Signaling Techonology
(1)
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Cell Signaling Technology
August 08, 2014
This antibody was used in a study that investigated the role of Phospho-p70 S6 Kinase (Thr389) in WT and transfected cardiomyocytes. Several...
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qRT-PCR Primers
(1)
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Integrated DNA Technologies
August 08, 2014
It is a great pleasure to write review on Integrated DNA technologies which provides qRT-PCR primers. We were screening expression of 500 ...
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Erba Mannheim Calcium Assay Kit
(1)
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August 07, 2014
I am working on phytochemicals in the area of renal research and I wanted to analyze the effect of phytochemicals on the extent of stone ...
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Erba Manheim SGPT Assay Kit
(1)
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August 07, 2014
As part of my work, I was in a search of a highly specific and sensitive protocol for estimation of SGPT as I am working on phytochemicals ...
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Erba Manheim Urea Assay Kit
(1)
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August 07, 2014
Urea is a key major end product of protein metabolism in humans. It constitutes the largest fraction of non-protein nitrogen component of ...
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Works for Most of Proteins
(1)
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Thermo Fisher Scientific
August 07, 2014
Gradient gels have several advantages for protein separation regarding the sharpness of bands and allowing to separate wider range of ...
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Simple Cloning for Protein Expression
(1)
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August 07, 2014
I've used DNA 2.0 to synthesize codon optimized genes for protein expression for over a dozen projects. Their service is very reliable. ...
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Application of PE Conjugated Anti Human MDR1 Ab
(1)
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BioLegend
August 07, 2014
We are trying to look at the expression of MDR1 in different cell lines by FACS. This Ab works great, as it can be directly used to stain ...
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Erba Manheim Creatinine Assay Kit
(1)
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August 07, 2014
Creatinine is a key waste product in muscle from high energy compound, creatine phosphate. The amount of creatinine produced is highly ...
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