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OxPhos Antibody Cocktail on Bone Marrow Macrophages and Osteoclasts
(1)
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Abcam
May 12, 2014
We aim to study whether a defect in NF-kB signaling results in defect in mitochondria oxidative phosphorylation subunit expression.
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Great SYBR Green Master Mix
(1)
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Bio-Rad
May 12, 2014
We have tested SsoAdvanced™ Universal SYBR® Green Supermix from Bio-Rad and always found this one to be superior (higher sensitivity, bright...
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High Transfection Efficiency: PC12 cells
(1)
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Lonza
May 09, 2014
My research work involved transfecting PC12 and differentiated PC12 cells to study the peripheral neuropathy after cisplatin treatments.
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Handy Western Blot: Good Results
(1)
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Thermo Fisher Scientific
May 09, 2014
I was looking for a way to speed up my western blots since I run many gels per week while doing a whole lot of other experiments.
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Acetylated-Lysine Antibody
(1)
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Cell Signaling Technology
May 09, 2014
Here, I showed the simple and easy way to detect modification of protein by acetylation.
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Solid Antibody for Detecting Endogenous mTOR
(1)
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Cell Signaling Technology
May 09, 2014
We use Cell Signaling's Rabbit mTOR antibody repeatedly in our lab's work studying the mTOR signaling pathway and its role as a target in ...
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DDB-2: Excellent Antibody
(1)
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Cell Signaling Technology
May 08, 2014
DDB2 is DNA Damage Binding protein. Its expression is downregulated in several cancers. We have been able to detect this protein very easily...
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BRDT Staining in Mouse Testis
(1)
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Abcam
May 08, 2014
This antibody was used to detect BRDT in mouse testis.
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FLI1/EWSR1 Aquarius FISH Probe from Cytocell
(1)
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Cytocell Ltd.
May 05, 2014
I am using the Cytocell FLI1/EWSR1 probe to assess gain or loss of these target regions in lymphoma samples. The probe can also be used to ...
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Powerful Microscope
(1)
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Evident
May 01, 2014
We are using this microscope for different types of application, such as cell imaging, immunofluorescence and animal in vitro imaging. It is...
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Excellent RAGE Antibody, Works Everytime
(1)
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Thermo Fisher Scientific
April 30, 2014
We aim to check the expression level of RAGE in neonatal rat brain. We just selected this antibody as it was the one having cheaper price ...
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Great Rb pA5 Antibody
(1)
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Abcam
April 29, 2014
Krt5 was used as an epithelial marker in mouse embryonic limb development and digit separation. Limbs were sectioned at 2um and ...
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Isolating Serum Exosomes Using ExoQuick
(1)
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April 28, 2014
I have used the ExoQuick solution to isolate exosomes from serum for subsequent downstream RNA extraction and qRT-PCR analysis. I previously...
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Human CD57 Antibody: Look No Further!
(1)
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BioLegend
April 23, 2014
Assessing the levels of CD57 on T cells is important as it has been shown to increase in diseases such as AIDS. We chose this product to ...
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Very Strong Signal: Anti-CAF-1 IF
(1)
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Santa Cruz Biotechnology, Inc.
April 22, 2014
The purpose of this experiment was to identify an antibody that was suitable for detection of the chromatin assembly factor CAF-1 in human ...
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SeraMiR Exosome RNA Purification Kit
(1)
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April 21, 2014
I have used the SeraMir Exosome RNA Purification kit to purify RNA from exosomes isolated from serum, using ExoQuick. I selected this ...
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P53/ATM Aquarius Hematology FISH Probe
(1)
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Cytocell Ltd.
April 18, 2014
I am investigating chromosomal aberrations in lymphoma and have used the P53/ATM probe to assess gain and loss of these targets from fresh-...
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Excellent Antibody to Detect Human MMP-13
(1)
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April 17, 2014
The goal of that study was to measure the protein amount of MMP-13 in human heart samples.
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Strong Signal: Anti-BRM IF
(1)
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Santa Cruz Biotechnology, Inc.
April 15, 2014
The purpose of this experiment was to identify an antibody that was suitable for detection of BRM in human cells by indirect ...
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Specific P62 Antibody
(1)
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Cell Signaling Technology
April 14, 2014
Highly specific p62 antibody
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The Only Machine I Would Ever Use For This Purpose !!!
(1)
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Eppendorf
April 11, 2014
Our lab does a lot of chromatin immuno-precipitations, 3C assays, immuno blots and other applications where you need to incubate the ...
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TIM-4 Expression of LPS Activated Peritoneal Macrophages
(1)
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BioLegend
April 11, 2014
To detect the TIM-4 expression on LPS activated peritoneal macrophages.
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StepOnePlus is a Step Above the Rest
(1)
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Thermo Fisher Scientific
April 11, 2014
Research in our lab focuses on how temperament or personality relates to physiology and health. The larger goal is to determine how and why ...
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Detection of Low Abundant Proteins by Chemiluminescent Western Blotting
(1)
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Advansta
April 08, 2014
We are interested in understanding the regulation of protein stability of those important low abundant proteins with short half-life in ...
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Anti-Brg-1: Good for Immunofluorescence
(1)
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Santa Cruz Biotechnology, Inc.
April 08, 2014
The purpose of this experiment was to identify an antibody that was suitable for detection of BRG-1 in human cells by indirect ...
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Strong Signal: Anti-BMI1 Immunofluorescence
(1)
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MilliporeSigma
April 07, 2014
The purpose of this experiment was to identify an antibody that was suitable for detection of BMI1 in human cells by indirect ...
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Love This Machine!
(1)
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Thermo Fisher Scientific
April 04, 2014
We typically perform 20-40 gene expression experiments each week. This machine is fantastic in that you can create templates, drop in your ...
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Good PCR Machine for a Low Price
(1)
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Corning Life Sciences
April 03, 2014
We bought this thermocycler to run 24/7 and it does the job , its not fancy with touch screens / graphics!! Does the job and that's the best...
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Strong Signal: Anti-BRCA1 Immunofluorescence
(1)
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Santa Cruz Biotechnology, Inc.
April 02, 2014
The purpose of this experiment was to identify an antibody that was suitable for detection of BRCA1 in human cells by indirect ...
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The Standard for Comparing All Other PCR Instruments
(1)
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Roche
April 02, 2014
Easy enough to train undergrads on, but powerful enough for a senior research scientist, the Roche LightCycler 480 is the gold standard I ...
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Very Good: Anti-ATRX, Immunofluorescence
(1)
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Santa Cruz Biotechnology, Inc.
March 31, 2014
The purpose of this experiment was to identify an antibody that was suitable for detection of ATRX in human cells by indirect ...
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Strong Signal: Anti-Acetyl-Lysine Immunofluorescence
(1)
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MilliporeSigma
March 28, 2014
The purpose of this experiment was to identify an antibody that could detect all acetyl-Lysine by immunofluorescence. Lysine acetylation is ...
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TCR Detection by Anti-TCRa/b
(1)
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BioLegend
March 26, 2014
My research focuses on expressing T cell receptors using lentiviral vectors. I use Jurkat cells to test the vectors for TCR expression. I ...
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PEI - An Inexpensive Transfection Reagent
(1)
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March 25, 2014
My research involves making lentiviral vectors pseudotyped with vesicular stomatitis virus envelop glycoprotein (VSV-G). In this protocol, I...
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The Best PCR Purification Kit of All Times
(1)
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March 24, 2014
As a TCR-immunotherapy project, a part of my research includes cloning various inserts (T cell receptors) into lentiviral vectors as well as...
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Antibody for HA Tagging a Protein
(1)
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March 21, 2014
Hemagglutinin (HA) epitope tag, or HA tag, is one of the most usual ways to mark and detect a protein in vivo. Good antibody for HA tag is ...
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Antibody for Ubiquitination Analysis
(1)
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MilliporeSigma
March 20, 2014
Ubiquitination is the most important way to regulate proteins in cells. Our lab has demonstrated that FbxL5 can direct SNA1 to degradation ...
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Good Results: Anti-SP3 and Anti-KLF6
(1)
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BioLegend
March 20, 2014
Purified anti-SP3 and purified anti-KLF6 antibodies give good results.
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V5 Tag Detection
(1)
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Thermo Fisher Scientific
March 19, 2014
Protein tagging is a very usual method to detect proteins with no available good commercial antibodies. V5 epitope tag is derived from a ...
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Easy and Fast Estimation of Glucose Uptake
(1)
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Cayman Chemical
March 18, 2014
Cayman's Glucose Uptake Cell-based Kit allows simple, fast and accurate estimate of uptake of a fluorescent glucose analog by cells. Results...
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Excellent Anti HSA (CD24) Antibody With Great Fluorescence
(1)
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BioLegend
March 17, 2014
Anti HSA antibodies have an annoying feature that when they bind the negative control jumps above unstained (this is not overcome by adding ...
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Ubiquitination in Nuclei by FBXL5
(1)
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Santa Cruz Biotechnology, Inc.
March 14, 2014
SNA1 is the main effectors of EMT due to its gene repression activity. Stabilization and subcellular localization of the protein plays a key...
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Cell Cycle Analysis. CDK1 Detection by WB
(1)
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March 13, 2014
We focus our research in EMT induced by SNA1 transcription factor. One of the poorly understood mechanisms is the effects on cell cycle ...
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CD14: Good Signal, but Relatively High Background!
(1)
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BioLegend
March 12, 2014
CD14 is the LPS receptor, expressed at high levels on monocytes. When staining ficoll-paque isolated human PBMC, the monocytic gate is not ...
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Excellent Antibody for T-Cell Activation
(1)
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March 11, 2014
My research focuses on T cell based immunotherapy. For this, I regularly activate and expand primary T cells from PBMCs. Anti-CD3 antibody ...
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Clathrin Heavy Chain Antibody
(1)
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Cell Signaling Technology
March 10, 2014
A clean antibody to detect Clathrin heavy chain. High specificity but sensitivity is okay, load 30-40 ug total protein to get a strong ...
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CD169 (Siglec-1) Antibody: Works Out of the Box
(1)
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BioLegend
March 07, 2014
The aim of the research is to study lymph node myeloid cells in cancer. In my experiment, I tested if a cytokine associated with tumor ...
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CD11b Antibody: Brilliant!
(1)
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BioLegend
March 06, 2014
The aim of the research is to study splenic hemopoietic progenitor cells in cancer. In my experiment, I tested if a cytokine associated with...
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CD169 Antibody: Reliable Reagent
(1)
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Bio-Rad
March 05, 2014
The aim of the research is to study lymph node myeloid cells in cancer. In my experiment, I tested if a cytokine associated with tumor ...
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CD40 Antibody: Excellent Product
(1)
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BioLegend
March 04, 2014
The product used to determine macrophage activation following TLR stimulation.
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