Excellent ab for iTreg population staining

December 08, 2014

Cancer Biology
Scripps research center in FL
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-mouse CD25 Antibody

Catalog Number:

102008

Info: View Product View Product Specs

Browse Similar Products: CD25 Antibodies

This Ab was used to stain mouse CD4 T cells differentiated iTreg in vitro including CD4+CD25+Foxp3+.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse cultured CD4 T cells

Primary Incubation

1:300 diluation, 15 minutes in ice

Blocking Agent

PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACS

Results Summary

Can stain mouse iTreg very brightly even at low concentration 1:300.

Additional Notes

None

Related Categories

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Summary

The Good

Easy usage even at low concentration 1:300, super bright.

The Bad

None

The Bottom Line

Can be easily used to stain mouse iTreg cultured in vitro.

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