Cell Signaling Technology
Phospho-Chk1 (S317)
2344S
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Chk1 is an effector kinase that acts downstream of ATR in response to DNA damage and replication stress. Phosphorylation of Chk1 at S317 and 345 are hallmarks of the activation cell cycle checkpoint. The Western blot analysis shown here was to to confirm the UV-induced phosphorylation of Chk1 pS317.
Western blot
HeLa cells
Dilution 1:1000 in 5% non-fat milk/TBS-Tween20. Two hour incubation at room temperature.
5% non-fat milk/TBS-Tween20
HRP-conjugated anti-rabbit IgG
NA
Bio-Rad ChemiDoc imager
The phosphorylation of Chk1 S317 was only observed in UV treated HeLa cells, but not the undamaged samples.
None
Super specific and sensitive
This is a great antibody with superb specificity and clean background.