Tonbo CD11c Red Fluor 710 antibody

DBS
University of Montana
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Tonbo Biosciences

Product Name:

Anti-CD11c Red Fluor 710

Catalog Number:

80-0114-U100

Browse Similar Products: CD11c Antibodies

We are assessing consequences of Aryl hydrocarbon Receptor (AhR) activation in dendritic cells. Specifically for these experiments we were assessing Aryl hydrocarbon Receptor activation in bone marrow derived dendritic cells. Precursor cells are isolated with Lympholyte M from whole bone marrow suspensions and grown in the presence of GM-CSF for 5-7 days. We then use flow cytometry and RT-PCR to assess changes in dendritic cells after AhR activation. Here, I used Tonbo CD11c for assessment of bone marrow-derived dendritic cells via fluorescence cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine BMDCs

Primary Incubation

1:100 at 4 degrees celsius for 25 minutes

Blocking Agent

FC Block

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

FACSAria II

Results Summary

Tonbo CD11c Red Fluor 710 is equivalent to other companies' antibodies. It works in the Alexa Fluor 700 channel.

Additional Notes

None

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Summary

The Good

Works as well as other CD11c antibodies

The Bad

Had a slight problem with compensation bead compatibility (had to use more than usual to get a strong positive population for compensation)

The Bottom Line

I would recommend this product. If using beads for compensation, you may need to use more antibody than usual.

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