Good IgM Antibody

CSB
MGH
postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC/Cy7 anti-mouse IgM Antibody

Catalog Number:

406516

Info: View Product View Product Specs

Browse Similar Products: IgM Antibodies

The aim of my research is to study lymph node cells in cancer. In my experiment, I tested if a cytokine associated with tumor promotion alters cell counts. We selected IgM Antibody from Biolegend specifically because IgM is expressed in B cells and can be found in lymph nodes. Using this product I was able to differentiate B cell subsets.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Lymph node

Primary Incubation

1:300 30' in ice

Blocking Agent

FcBlock

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Fluorescence

Results Summary

Target expression is very low (~1 log) so need to optimize PMT settings of the channel (and thus of nearby channels)

Additional Notes

None

Image Gallery

Summary

The Good

Important reagent to have

The Bad

Target is dim

The Bottom Line

Useful to have

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