Menin Antibody for Western Blotting

Patholgoy
University of Michigan
Post Doc Fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Bethyl Laboratories

Product Name:

Menin Antibody

Catalog Number:

A300-105A

Info: View Product View Product Specs

Browse Similar Products: Menin Antibodies

We were interested in looking at the protein levels of menin as well as its interacting partners in prostate cancer cell lines (VCaP and LNCaP). We performed immunoprecipitation with menin antibody followed by Western blotting using other antibodies. Bethyl menin antibody performs really well for IP as well as Western blotting. It is very consistent between various batch as well.

Experimental Design and Results Summary

Applications

Immunoprecipitation

Sample

VCaP and LNCaP cell extracts

Primary Incubation

3ug for IP and 1:1000 for WB

Blocking Agent

5% Milk

Secondary Incubation

1:5000 anti Rabbit from Jackson Lab for 30 min in 5% Milk

Tertiary Incubation

NA

Detection

Thermo Fisher detection agent

Results Summary

This antibody perfromed really well for both IP and WB. We have published results obtained in out latest research publication. PMID: 25822367

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Very consistent between different batches

The Bad

Not good for ChIP-seq

The Bottom Line

One of the best menin antibodies if you want to do IP and WB.

Share your experience with other scientists. Write a Review! »

Join the discussion