Isolation of RNA from T cells

June 12, 2015

University of Nebraska Medical Center
Pharmacology and Experimental Neuroscience
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Qiagen

Product Name:

RNeasy Mini Kit

Catalog Number:

74104

The purpose for using this kit was to isolate RNA from a small number of T cells. I chose it because it seemed easy to use, quick, and it allowed me to isolate RNA from a small starting sample.

Experimental Design and Results Summary

Application

RNA isolation

Starting Material

T cells isolated from spleens and lymph nodes

Protocol Overview

I freshly isolated T cells from drug-treated mice and used this kit to isolate RNA from them. The kit came with an easy to follow protocol and all the necessary buffers, columns, and collection tubes. However, I did choose to add a DNAse in order to rid my sample of DNA contamination.

Tips

I needed to get a bag of extra collection tubes because there were not quite enough with the kit.

Results Summary

I was able to isolate high quality RNA from a small amount of T cells (1 million) to use for future application in PCR.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Cheap, easy to optimize, isolates quality RNA

The Bad

None

The Bottom Line

The kit has simple directions and an easy to follow protocol. It also allows isolation of RNA from a small starting sample, and it comes with everything that you need. I would recommend its use to others.

Join the discussion