GeneTex
Human IgG1 Antibody
GTX63185
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I used this antibody to screen my protein of interest in culture supernatants which had an IgG1 Fc tag. Earlier, with other anti IgG antibodies I was getting many non-specific bands. To overcome that I tried this IgG1 specific antibody.
Western Blot
Cell culture supernatant from transfected cells
1:2000 for 3hr at room temperature
5% Skimmed milk
1:5000 anti-rabbit-HRP
NA
ECL
I got a specific band at expected size (170kda) without any background.
None
Good specificity and ease of optimization
Expensive
We can use this IgG subclass antibody for specific experiments which require sub-type specificity.