CyclinB1 is a very good antibody for Western blot with out any non specificicity

June 11, 2015

chemical biology
Indian institute of chemical technology
SRF

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Quality of Results

Ease-of-Optimization

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Company:

Sigma-Aldrich

Product Name:

CyclinB1 Anti-rabbit antibody

Catalog Number:

SAB4503501

Browse Similar Products: Cyclin Antibodies

This product was used in a study to screen anticancer drugs that can inhibit G2/M phase of the cell cycle, in this case CyclinB1 is used as a positive controle for Western blotting.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Hela cell lysate,A549 Cell lysate

Primary Incubation

1:1000, For 2hr at room temperature, or overnight at 4 degreees

Blocking Agent

Skim milk for 1hr at RT

Secondary Incubation

1:5000 dilution, incubation for 1 hr, anti-rabbit

Tertiary Incubation

NA

Detection

ECL chemiluminiscent reagent

Results Summary

This antibody is very senitive and we produced good, publishable quality blots.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Highly sensitive, stable at 4 degrees

The Bad

None noted

The Bottom Line

Developing good blots with this antibody is not difficult and there is no non specific binding.

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