Alpha tubulin expression in HELA cells

June 09, 2015

chemical biology
Indian institute of chemical technology
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Company:

Sigma-Aldrich

Product Name:

Alpha tubulin

Catalog Number:

T6074

Browse Similar Products: Alpha-Tubulin Antibodies

This product was used as part of a study to check protein expression changes in cancer. The product worked well as a loading control.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HELA cell lysate

Primary Incubation

1:3000, 2h room temperature

Blocking Agent

1h skim milk in 1XTBST(0.1%Tween-20)

Secondary Incubation

Anti-mouse-HRP, 1:5000, 1h Room temperature

Tertiary Incubation

No tertiary incubation

Detection

Dark room with x-ray film

Results Summary

The expresion of alpha tubulin is the same as in samples with equal amount of protein.

Additional Notes

None

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Summary

The Good

Highly sensitive, stable at 4C

The Bad

If lower dilution is used, there is a chance of nonspecificity

The Bottom Line

I would recommend for use as a loading control because it is highly sensitive and shows less non-specificity than other options

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