H3 pan as loading control for checking histone modifications

chemical biology
indian institute of chemical technology

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Company:

EMD Millipore

Product Name:

Anti-Histone H3, PAN

Catalog Number:

05-928

Info: View Product View Product Specs

Browse Similar Products: Histone H3 Antibodies

We do work on gene and protein expression changes (epigenetic modulators) in neuroglia differentiation and behaviour. We used this product to check the histone acetylation in glioblastoma cells treated with anticancer drugs.

Experimental Design and Results Summary

Applications

Western Blot

Sample

GL261

Primary Incubation

1:500, Overnight, 4c

Blocking Agent

5%BSA in 1xTBST

Secondary Incubation

Rabbit HRP

Tertiary Incubation

No tertiary incubation

Detection

Chem doc

Results Summary

The H3 PAN levels are equal in wells loaded with equal amount of protein.

Additional Notes

None

Related Categories

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Summary

The Good

High signal with good specificity

The Bad

Cost

The Bottom Line

Can be used as a loading control to check the histone modifications

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