Life Technologies
Hoechst 33342, Trihydrochloride, Trihydrate
H3570
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Primary tissues and cells are routinely analyzed for various biochemical and pharmacological behavior. One of the primary methods to quantify and identify functional behavior is to stain the nucleus to locate intracellular compartments. Hoechst 33342 performed this task with remarkable ease and can be used to stain a wide variety of cells.
Immunofluorescence, Flow Cytometry, Confocal Microscopy
Mice primary cells in suspension or frozen tissue sections
A 100-fold diluted working solution of the stock was prepared in common buffers such as PBS or HBSS. Cells or tissues were incubated with volumes ranging from 50-200 ul of the working solution for 15 minutes at room temperature in the dark. The samples were then immediately visualized under cell-culture conditions or fixed and mounted for later visualization. Visualization can be done using fluorescence channels routinely employed for DAPI.
Do not use the dye more than what is needed or incubate for longer as it can lead to easily saturated images. Hoechst diffuses and binds rather quickly
The clear nuclear staining was seen within minutes of staining and was also very uniform across the entire sample. There was no bleed-through in other fluorescence channels.
None
Ease of Use. Stable for a long period of time in the fridge.
None so far
Excellent Live-Cell Nucleus Stain for live-cell tracking and counterstaining. Not a viability marker.
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