Life Technologies
Goat anti-rabbit alexa 488
A-11070
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We study sinus disease in humans, and specifically we were looking at epithelial tight junction staining in ex vivo cultured epithelium.
Immunofluorescence
Normal human bronchial epithelial cells
1h at RT
10% goat serum
1:500 dilution, 1h at RT
NA
This secondary antibody has always worked well for us with many different primary antibodies. The staining is always very bright and one vial lasts a long time.
Reference: Oncostatin M promotes mucosal epithelial barrier dysfunction, and its expression is increased in patients with eosinophilic mucosal disease. Pothoven KL, Norton JE, Hulse KE, Suh LA, Carter RG, Rocci E, Harris KE, Shintani-Smith S, Conley DB, Chandra RK, Liu MC, Kato A, Gonsalves N, Grammer LC 3rd, Peters AT, Kern RC, Bryce PJ, Tan BK, Schleimer RP. J Allergy Clin Immunol. 2015 Apr 1. pii: S0091-6749(15)00188-8. doi: 10.1016/j.jaci.2015.01.043. [Epub ahead of print] PMID: 25840724
Reliable antibody
None
Use this secondary antibody