Cell Signaling Technology
Histone H3 antibody
9715
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We carry out subcellular fractionation and verify the isolated fractions by western blot analysis. We have had good experience with Cell Signaling Technology antibodies and so use histone H3 as a nuclear marker.
Western Blot
Rat liver tissue and hepatoma cell line
1:2000 overnight at 4C
1:2 Odyssey buffer:TBS with 0.1% tween20
1:2 Odyssey buffer: TBS 0.1% tween20, 0.02% SDS
NA
LiCor Odyssey
We see enrichment of histone H3 in the nuclear fraction (lane 3) compared to total lysate (lane 2). The band migrates slightly below the predicted size of 17 KDa (lane 1).
None
Reliable, sensitive
No major drawbacks
It produces a consistently good signal and works well with Odyssey including multiplexing with other antibodies in our experience.