Good HRP Conjugated Beta-Actin Antibody

Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

β-Actin Antibody (C4) HRP

Catalog Number:

sc-47778 HRP

Info: View Product View Product Specs

Browse Similar Products: Beta-Actin Antibodies

I use this antibody as an internal control for my quantitative blots. Initially, I was using a beta-actin antibody from another supplier when I needed a secondary. To save time and improve the quality of my re-probed blots I prefer this antibody. It works remarkably well.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse CD4+ T cells

Primary Incubation

1:10000 for 1h at room temperature

Blocking Agent

5% Milk

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

ECL

Results Summary

I am getting a very clear band without any non-specific bands with a very short exposure time, within seconds. This is a very good choice as an internal control.

Additional Notes

None

Related Categories

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Summary

The Good

Specific band and time saving

The Bad

Not much except cost

The Bottom Line

Great choice for an internal control antibody

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