Lonza
BEGM BulletKit
CC-3170
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We use this media to grow bronchial and nasal epithelium at air-liquid interface conditions. The media is very easy to make, you just add the individual supplements, 9 total, to the media and sterile filter. The media is good for about a month, just keep in mind that it should be kept from the light because there is retinoid acid in the supplement kit.
Cell culture
Airway epithelial cells
Epithelial cells are seeded onto transwell plates with 1mL BEGM in the bottom chamber and .5mL in the top. The media is changed every other day until the cells are confluent on the transwell membrane. The media is then taken out of the top chamber and the cells are transitioned into air liquid interface. The media is changed every 2-3 days until the cells are fully differentiated around day 21.
Keep the media away from the light as much as possible because it contains retinoid acid.
Epithelial cells grown in this media become fully differentiated and develop barrier function, as shown by the tight junction staining pictured.
Very easy to make the media
Reference: Oncostatin M promotes mucosal epithelial barrier dysfunction, and its expression is increased in patients with eosinophilic mucosal disease. Pothoven KL, Norton JE, Hulse KE, Suh LA, Carter RG, Rocci E, Harris KE, Shintani-Smith S, Conley DB, Chandra RK, Liu MC, Kato A, Gonsalves N, Grammer LC 3rd, Peters AT, Kern RC, Bryce PJ, Tan BK, Schleimer RP. J Allergy Clin Immunol. 2015 Apr 1. pii: S0091-6749(15)00188-8. doi: 10.1016/j.jaci.2015.01.043. [Epub ahead of print] PMID: 25840724
Great media for differentiating airway epithelium at air liquid interface
Expensive
If you want to grow airway epithelium at air liquid interface, you should use this media.
Product Name: BEGM™ Bronchial Epithelial Growth Medium BulletKitSupplier Page