Western Blot Analysis of FASN

Biology
University of Houston
Graduate student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Santa Cruz Biotechnology, Inc.

Product Name:

Fatty Acid Synthase (A-5) Antibody

Catalog Number:

sc-55580

Info: View Product View Product Specs

Browse Similar Products: FASN Antibodies

Our laboratory is focused on studying the role of EGFR in de novo fatty acid synthesis and mitochondrial dynamics. This product was used to detect FASN through Western blotting.

Experimental Design and Results Summary

Applications

Western Blot

Sample

PC3 cell lysates

Primary Incubation

1:3000, overnight at 4 C

Blocking Agent

5% milk

Secondary Incubation

1:3000, one hour at room temperature

Tertiary Incubation

NA

Detection

ECL and film

Results Summary

Very good antibody for western blotting, ICC, IF and immunoprecipitation.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Very easy to detect FASN with this antibody compared to other sources

The Bad

None

The Bottom Line

Works very well for WB, ICC, IF and IP with low background.

Share your experience with other scientists. Write a Review! »

Join the discussion