Santa Cruz Biotechnology Inc.
FGF-2 Antibody (147)
sc-79
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Proteins were extracted from mammalian cell lines treated with growth factor. Equal amounts of proteins were resolved in 12% SDS-PAGE then western transferred and probed with FGF2 antibody supplied from Santa Cruz Biotech Inc.
Western Blot
Whole cell lysate
1:500 (5% Skimmed milk in TBST), Overnight at 4 degrees Celsius
5% Skimmed milk in TBST (2h)
1:4000 (5% skimmed milk in TBST), 1h at RT
NA
ECL
This antibody from Santa Cruz Biotechnology, Inc. showed very bad detection of FGF2 in our mammalian cell line. Lots of non-specific bands with high intensity were detected. The above antibody does not comply with the results as claimed in the antibody flyer.
None
Lots of non-specific bands
Bad antibody