Concentrating DNA in Very Small Volume

The Jackson Laboratory
Cancer biology
Post doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Zymo Research

Product Name:

DNA Clean & Concentrator

Catalog Number:

D4013

We used this kit because we need to concentrate the plasmid DNA in a smaller volume for transfection.

Experimental Design and Results Summary

Application

Concentrating DNA

Starting Material

Plasmid DNA

Protocol Overview

The low concentrated plasmid DNA (200-300 ul) was added with 3 times of the binding buffer and remaining steps followed the kit manual.

Tips

Increase the binding buffer ration

Results Summary

We used get concentrated DNA least 5 fold in the least 10ul volume.

Additional Notes

None

Image Gallery

Summary

The Good

Small elution volume

The Bad

20-30% loss

The Bottom Line

Very good for concentrating DNA in small volume with shortest time.

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