DNA Ligation Kit

University of Miami
Cell Biology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

New England Biolabs

Product Name:

T4 DNA Ligase Kit

Catalog Number:

M0202T

This is a good kit for DNA ligation. Good for ligating sticky and blunt ends with overnight incubation. Ligated DNA successfully transforms into bacteria.

Experimental Design and Results Summary

Application

Molecular Cloning

Starting Material

None

Protocol Overview

Mix DNA and ligation reagents. Incubate overnight in the fridge. Heat inactivate and transform ligated DNA.

Tips

Heat Inactivate

Results Summary

After overnight incubation, the ligated DNA successfully transformed into competent cells.

Additional Notes

None

Image Gallery

Summary

The Good

Works

The Bad

Not as quick as other kits

The Bottom Line

This is a good kit for ligating DNA.

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