B220 Expression on Spleen Cells in BALB/c

April 22, 2016

Immunology
University of Rochester
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

b220 anti-mouse BV711

Catalog Number:

103255

B220 was used to gate on B cells in the spleen.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lymph node

Primary Incubation

30min on ice. Used manufacturer's concentration.

Blocking Agent

FcBlock

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Fortessa

Results Summary

B220 costained with another B cell marker CD19. It is bright enough to distinguish from negative population.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Bright

The Bad

Use a lot of antibody for staining.

The Bottom Line

Excellent antibody.

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