CD11c Staining on DCs

April 25, 2016

Immunology
University of Rochester
Graduate Student

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Company:

BioLegend

Product Name:

cd11c percp cy5.5 anti-mouse

Catalog Number:

117328

This antibody was used to distinguish DCs from spleen cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Spleen cells BALB/c

Primary Incubation

30min on ice. Used manufacturer's concentration.

Blocking Agent

Fc Block

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Fortessa

Results Summary

CD11c is not as bright as expected. It is low, but can gate on the cells.

Additional Notes

None

Related Categories

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Summary

The Good

Can distinguish cells

The Bad

Low staining and uses a lot of antibody.

The Bottom Line

Staining is low.

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