Expression of CD123 on Mouse DCs

April 19, 2016

BlueskyReddit
Immunology
University of Rochester
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

eBioscience

Product Name:

CD123 anti-mouse APC

Catalog Number:

17-1231-82

This antibody was used as a preliminary experiment to assess surface CD123 expression on naïve DCs in the lymph node.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lymph node cells from BALB/c

Primary Incubation

30 min on ice. 1.25ul per 100ul test.

Blocking Agent

FcBlock

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Fortessa

Results Summary

Expression is not bright. A small population is seen to express low levels compared to isotype.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Ease of use

The Bad

Not bright

The Bottom Line

Ok to use to assess this marker. However, expression is dull.

Share your experience with other scientists. Write a Review! »

Join the discussion