Cell Signaling Technology
Acetyl-alpha-tubulin (Lys40)
#5335
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Browse Similar Products: Acetyl-alpha-Tubulin Western Blot Antibodies
Acetylated tubulin has been implicated in ER localization and ER-mitochondrial interactions and many other functions associated with cellular homeostasis. This antibody was chosen to assess the amount of acetylated tubulin in response to a number of cellular stresses.
Western Blot
Total cell lysates of rat H4IIE hepatoma cells
1:500 antibody in 0.1% tween and 1:3 odyssey block: 1X TBS at 4 degrees C overnight.
1:3 odyssey block:1X TBS
1:15,000 licor IRDye 680RD secondary antibody in 0.1% tween, 0.02% SDS, and 1:3 odyssey block: 1X TBS
NA
Licor Odyssey Infrared Imaging System
Antibody works in H4IIE rat hepatoma cells and shows a distinguished band at 55kDa. Recommended dilution was spot on.
None
Inexpensive compared to others. Clean blot. Minimal background.
Nothing!
Recommended dilution works well and blot is clean with minimal background. Easy to multiplex with total tubulin or other antibodies.