Good Antibody for Excluding Endothelial Cells

Department of Urology
UT Southwestern Medical Center
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

a-Hu CD31 V450

Catalog Number:

561653

Flow cytometry was used to probe epithelial dynamics and the immune infiltrates in human prostate tissues. Epithelia was investigated with CD45-CD325+ cells. Leukocytes were looked at using CD326-CD45+. Sub-populations (such as epithelial stem cells or individual leukocyte populations like T-Cells) were gated with appropriate markers. This viability dye was used in the same color to exclude non-viable and endothelial cells at the same time.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Primary human prostate cells

Primary Incubation

Recommended for 20-30mins

Blocking Agent

FcX

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow Cytometer

Results Summary

Viable, non-endothelial = V450-

Additional Notes

None

Related Categories

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Summary

The Good

Easy, no surprises

The Bad

Expensive

The Bottom Line

Buy if you want to exclude endothelial cells and are using a viability dye in V450

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