Great CD303 for PE-Texas Red Channel

Department of Hematology/Oncology
MSSM
Associate Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

CD303 PE-Vio615

Catalog Number:

130-107-454

The general aim of our research is to improve the study of human dendritic cells. In order to do so, we have developed a system to produce different DC subsets using a specific culture and cytokine mix. In order to identify pDCs, CD303 and CD123 are used.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Differentiated myeloid cells from CD34 (cord blood)

Primary Incubation

1:200, 30 minutes at 4deg

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry (LSR Fortessa)

Results Summary

pDCs were positively identified as lineage- HLA-DR+, CD141-, CD1c-, and CD45RA+

Additional Notes

None

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Summary

The Good

Everything! Great use of PE-Texas RED

The Bad

Nothing!!

The Bottom Line

Awesome antibody to use...

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