Great, Reliable Mouse IL-17 DuoSet ELISA

University of California, San Diego
Department of Pediatrics
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D Systems

Product Name:

Mouse IL-17 DuoSet ELISA, 5 Plate

Catalog Number:

DY421

This product was used to detect cytokines in murine lung homogenate. This is a consistent, reliable ELISA.

Experimental Design and Results Summary

Application

ELISA

Starting Material

Murine lung homogenate supernatant.

Protocol Overview

Lungs from Mtb infected mice were homogenized in 5mL PBS-T. Homogenate was aliquoted and spun down at max speed, and the supernatant was filtered through a 0.2um PVDF filter. Sample was diluted 1:3 in the appropriate buffer as per product guidelines

Tips

May need to dilute sample to get within the standard curve.

Results Summary

Consistent, reliable ELISA

Additional Notes

None

Image Gallery

Summary

The Good

Works consistently

The Bad

None

The Bottom Line

Reliable and easy to use, will use again.

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