Great Use of Alexa Fluor 700 Channel

Department of Hematology/Oncology
MSSM
Associate Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BD Biosciences

Product Name:

HLA-DR APC-R700

Catalog Number:

565127

We isolated CD34+ cells from cord blood, and then used a specific media+cytokine mix to isolate a high yield of dendritic cells. HLA-DR was used as a marker to identify dendritic cells in the population.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Differentiated myeloid cells from CD34 (cord blood)

Primary Incubation

1:500, 30 minutes, 4deg

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry (LSR Fortessa)

Results Summary

We were able to identify both an HLA-DR high and an HLA-DR intermediate population using this antibody when gated on live CD45+ cells.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Works much better than an HLA-DR Alexa Fluor 700 AB

The Bad

None

The Bottom Line

Great antibody!

Share your experience with other scientists. Write a Review! »

Join the discussion