Santa Cruz Biotechnology, Inc.
HDAC7 (A-7) Antibody
sc-74563
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To study the mechanisms of endothelial-to-mesenchymal transition. This particular experiment was to detect the expression of HDAC7 in human coronary artery endothelial cells before and after knockdown with shRNA. HDAC7 shRNA and antibody were both purchased from Santa Cruz Biotech.
Western Blot
Lysed Human Coronary Artery Endothelial Cells
1:500 dilution in 5% skim milk incubated at 4 degrees celcius overnight
5% skim milk for 1 hour at room temperature
5% skim milk with Goat anti-mouse IgG conjugated to HRP. 1:15,000 dilution for 2 hours at room temperature
NA
Enhanced chemiluminescence for 2 minutes
Clear bands at expected molecular weight of 105kDa. Lane 1/2 is scrambled shRNA control, lane 3/4 shows knockdown of HDAC7 protein using HDAC7 shRNA. 25ugs of protein loaded into each well after 5 minutes of boiling in laemmli buffer. Imaged on a Biorad western blot imager.
None
Easy and clear band visible with very short exposure time
Since good HDAC7 antibodies are very difficult to find, this one was very good.