Boster
Rabbit Anti-Human CD44 Picoband Antibody
PA1021-2
Browse Similar Products: CD44 Western Blot Antibodies
The antibody was used to detect the full length and cleaved fragments of human transmembrane protein CD44. The rabbit antibody PA1021-2 is sensitive, i.e. detects CD44 protein bands under reducing conditions and also when tested material is loaded at low total protein per lane. The antibody is highly specific, i.e. does not recognize any bands of unknown nature on the membrane.
Western Blot
Tumor cell lysate
Overnight at 8-10 degrees Celcius, with rocking, 1 ug/ml in 5% milk/BPS/Tw
5% milk in PBS/0.05% Tween-20 (5% milk/PBS/Tw)
Goat anti-Rabbit antibody conjugated with HRP at 1:3,000 in 5% milk/PBS/Tw
HRP-bound secondary antibodies were detected by WestPico from ThermoScientific
Chemiluminescence: West Pico from Thermo Scientific
The antibody recognizes the expected ~80 kDa full-length CD44 and its low mol. wt. fragments containing C-terminal domain. The antibody is highly specific, produces "clean", definitive results; does not produce any non-specific bands. The antibody is sensitive and detects CD44 band when total protein per lane is loaded at 10-20 ug. The antibody is stable and could be re-used for blotting several times when stored in the original 5% milk/PBS/Tw solution at -20oC.
None
High specificity and stability
None noticed
The antibody PA1021-2 is excellent for detecting human CD44 containing C-terminal domain.