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Date Published
A Reliable Antibody for P-EIF4E Check with Western Blot
(1)
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Cell Signaling Technology
May 23, 2016
Antibody Phospho-eIF4E (Ser209) is used for detecting endogenous levels of eIF4E only when phosphorylated at serine 209. eIF4E binds to ...
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Good Staining in Flow Cytometry with Mouse Monoclonal (C8/468 + C8/144B) to Human CD8 alpha
(1)
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Abcam
May 20, 2016
The main area of focus of our lab is lymphocyte biology, with a focus on T cell biology. Our goal in using this product was to identify CD8+...
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T-bet Antibody Gives Good Staining
(1)
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BioLegend
May 20, 2016
The aim is to determine how Tbet expression changes in T cells after different stimulations. This product was selected because Biolegend is ...
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2D Starter Kit
(1)
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Bio-Rad
May 20, 2016
This kit is good and saves time when running 2nd gels.
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Great Antibody for Looking at the Kinetochore
(1)
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May 20, 2016
This antibody marks the kinetochore really well. A great antibody for counting lagging chromosomes. Super strong, very specific, and easy to...
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A Reliable Antibody for Claudin-2 Protein Check
(1)
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Thermo Fisher Scientific
May 20, 2016
This is a mono-antibody, the dilution used was usually from 1:500, and dilution was increased based on the background. This is a good ...
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Staining of Mouse Colorectal Tumor Tissue
(1)
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BioLegend
May 20, 2016
This antibody was used to analyze the expression of ß Catenin in intestinal tumor cells. Intestinal tumor tissue was collected from Min+ ...
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Anti-Mouse HRP
(1)
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Thermo Fisher Scientific
May 20, 2016
We use this product as a secondary antibody to ELISA and WB, using a primary antibody mouse anti-HIS. We choose this company and this ...
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CD11c_APC-Fire750
(1)
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BioLegend
May 19, 2016
This is a great replacement for APC-Cy7 or APC-H7 for flow cytometry, it works well and as advertised.
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FITC-Conjugated Monoclonal Mouse Anti-Human CD3 Antibody (for Flow Cytometry) from BioLegend (Clone SK7)
(1)
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BioLegend
May 19, 2016
We used this antibody to identify CD3+ T cells from human peripheral blood mononuclear cells (PBMC) by flow cytometry. The antibody ...
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Time Course Effect of Chemoattractant on Pak2 Phosphorylation
(1)
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Cell Signaling Technology
May 19, 2016
Pak2 is a family of serine/threonine protease kinase which acts as a targets for small GTP binding proteins and have been implicated in ...
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RNA Purification
(1)
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EPICENTRE Biotechnologies
May 19, 2016
Isolation and handling of ribonucleic acid (RNA) is more difficult than DNA due to the presence of ubiquitous RNases. Purification of high-...
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A Reliable Antibody for Immunohistochemistry Staining on Gl Syn
(1)
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Santa Cruz Biotechnology, Inc.
May 19, 2016
This is a very strong antibody and gives clear staining. The background can be reduced when increasing the dilution of primary antibody. The...
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Staining of Mouse Intestinal Tissue
(1)
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BioLegend
May 19, 2016
To analyze the intestinal tumor cells, Min+ mice were sacrificed and tumor polyps from intestinal tissue were collected and snap frozen. ...
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An Essential Piece of the MeCP2 Antibody Tool Kit
(1)
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May 19, 2016
Our lab is interested in how Mecp2 (the gene/protein linked to Rett Syndrome) functions within the brain and how loss of function creates ...
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Isolating CD4+ Lymphocytes from Peripheral Blood Cells
(1)
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May 18, 2016
While there are a number of methods to separate/sort out lymphocytes from peripheral blood cells, isolating CD4+ specifically with a high ...
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Time Course Effect of Chemoattractant on ABCA1 Expression
(1)
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Abcam
May 18, 2016
ATP-binding cassette transporter is a transporter which regulates the cellular cholesterol and phospholipid homeostasis. Here we checked the...
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iBind Western
(1)
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Thermo Fisher Scientific
May 18, 2016
The goal of this experiment was to see if the iBind device worked effectively and efficiently.
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Countessa FL - Unattributed Review*
(1)
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Thermo Fisher Scientific
May 18, 2016
This was used for transduction of cells using LV and analysis of GFP expression using the Countessa FL. *Reviews submitted to our site ...
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Cholesterol-Lipid Analyzer
(1)
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May 18, 2016
This instrument is a diagnostic tool, use to test for cholesterol and lipids profile, that provide information about heart disease, ...
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FITC-Conjugated Monoclonal Mouse Anti-Human CD3 Antibody (for Flow Cytometry) from BioLegend (Clone OKT3)
(1)
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BioLegend
May 18, 2016
This antibody was used to identify CD3+ T cells in human peripheral blood mononuclear cells (PBMC) by flow cytometry. Bright staining was ...
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Large High Quality Plasmid DNA Kit in Nominal Cost
(1)
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Promega
May 18, 2016
Our lab regularly uses promega midi prep kit for large scale plasmid isolation from 100-200ml bacterial culture for transfection. We ...
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A Reliable Antibody for Immunohistochemistry Staining on GS
(1)
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Santa Cruz Biotechnology, Inc.
May 18, 2016
This is a poly-antibody from Santa Cruz Biotechnology; the dilution for IHC is about 1:50 to 1:100 in accordance with the IHC protocol.
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DNA Extraction from Soil Samples
(1)
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Mo Bio Laboratories, Inc.
May 17, 2016
I am working on the development of molecular techniques for the detection of bacteria in the environmental samples. The PowerSoil ® DNA ...
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Monoclonal Mouse Anti-Human CD3 Antibody (for Flow Cytometry) from BioLegend (Clone HIT3a)
(1)
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BioLegend
May 17, 2016
This antibody was used to identify CD3+ T cells in human peripheral blood mononuclear cells (PBMC) by flow cytometry. This antibody gave ...
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Trypsin IGD Kit
(1)
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May 17, 2016
The enzyme Trypsin is found in the digestive system where it hydrolyses proteins. Trypsin cleaves peptide chains mainly at the carboxyl side...
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Easy Detection of Mouse IL-13 Levels
(1)
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May 17, 2016
This kit works very well as it has good sensitivity and reproducibility.
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A Reliable Antibody for Western Reference Gene
(1)
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Santa Cruz Biotechnology, Inc.
May 17, 2016
This antibody was used at 1:1000 dilution in 5% BSA incubation overnight, it's a poly-antibody and should stored at 4 degrees.
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A Good Protein Gel Staining Kit
(1)
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May 17, 2016
Protein gels are stained with silver staining kit to confirm the quantity and quality of protein purification.
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Best Bicinchoninic Acid (BCA) Protein Quantification
(1)
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G Biosciences
May 17, 2016
Determination of protein concentration is essential for molecular assays in bioscience. The BCA assay from G bioscience is very easy to ...
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Miltenyi CD4 Microbeads, Human - Reliable Method for the Magnetic Positive Selection of CD4+ T Cells from Human PBMC
(1)
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Miltenyi Biotec
May 16, 2016
Several of our in vitro experiments require purified primary CD4+ T cells . We have worked with total PBMC in the past, but found that using...
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Anti-Mouse CD3 PECy7
(1)
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BioLegend
May 16, 2016
This is a good anti-mouse CD3 antibody. The experiments were performed using mouse splenocytes stimulated in vitro for 3 days with 0.5ng of ...
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Epithelial Progenitor Marker
(1)
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BD Biosciences
May 16, 2016
Flow cytometry was used to probe epithelial dynamics and the immune infiltrates in human prostate tissues. Epithelia was investigated with ...
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Excellent Anti Phospho-S6 Ribosomal Protein Antibody
(1)
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Cell Signaling Technology
May 16, 2016
I aimed at detecting Phospho-S6 Ribosomal Protein after IGF stimulation in a human cell line. This antibody was selected based on the good ...
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Western Blot ECL Substrate
(1)
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May 16, 2016
This kit has somewhat high sensitivity and is durable, less antibody and substrate can be used with this kit.
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Apoptosis Detection with Annexin V/Propidium Iodide
(1)
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May 16, 2016
While there exists a plethora of methods to detect cellular apoptosis, the Annexin V/Propidium Iodide is a method that has been highly ...
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DNA Synthesis Service Provided by Sigma-Aldrich
(1)
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May 16, 2016
I ordered a thiol group functionalized 25mer with a randomized sequence from Sigma-Aldrich. The starting materials were 250 nmol and I got ...
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Anti-Human CD14 Microbeads – Quickly Obtain Highly Pure CD14+ Monocytes/Macrophages from PBMC
(1)
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Miltenyi Biotec
May 13, 2016
Our lab requires highly pure immune cell subsets, such as monocytes and macrophages, for our experiments. To purify monocytes and ...
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Good Anti-Mouse CD69 Antibody
(1)
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BioLegend
May 13, 2016
Good antibody anti-mouse CD69. The experiments were performed using mouse splenocytes stimulated in vitro for 3 days with 0.5ng of OKT3. ...
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Sensitivity of Kit in Extraction of DNA
(1)
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QIAGEN - Sample to Insight
May 13, 2016
I am working on the development of molecular techniques for the detection of bacteria in the environmental samples.The QIAamp DNA Mini kit ...
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Excellent B-Cell Marker
(1)
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Tonbo Biosciences
May 13, 2016
Flow cytometry was used to probe epithelial dynamics and the immune infiltrates in human prostate tissues. Epithelia was investigated with ...
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Review for Dionex DNAPac PA100 Nucleic Acid Column
(1)
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May 13, 2016
An anion exchange column (AEC) (Dionex®DNAPac PA100 Nucleic Acid Column, 4 x 250 mm) was used for the purification of DNA tetrahedral ...
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APC Mouse IgG1, kappa Isotype Ctrl for Flow Cytometry from BioLegend
(1)
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BioLegend
May 13, 2016
Isotype control used in combination with Biolegend product # 313206 to account for non-specific in human synovial cells yielded by enzymatic...
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A Reliable Antibody for CCND1 Check With Immunohistochemistry Staining
(1)
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May 13, 2016
I used this antibody for immunohistochemistry staining, the dilution is 1:100 or 1:200 depending on CCND1 expression level in the tissue.
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Good Brachyury Antibody for Immunofluorescence in Human Cells
(1)
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R&D Systems, a Bio-Techne Brand
May 12, 2016
The aim of this experiment was to detect mesodermal cells derived from human iPS cells.
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Anti-Human CD8 Microbeads - A Consistent and Reliable Method to Positively Select CD8+ T Cells from PBMC
(1)
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Miltenyi Biotec
May 12, 2016
Our lab routinely works with purified immune cell subsets from human peripheral blood mononuclear cells (PBMC). In order to obtain highly ...
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Thermo p21 Antibody (701151) Never Works
(1)
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Thermo Fisher Scientific
May 12, 2016
I tested p21 expression level in Huh7 cells. After treating doxorubicin, p21 is up-regulated, which is confirmed by RT-qPCR. Compared to the...
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A Reliable Antibody for P-Akt 473 Expression Detection
(1)
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Cell Signaling Technology
May 12, 2016
This is a rabbit host antibody, and it is specific for detecting endogenous levels of Akt1 only when phosphorylated at Ser473. It also ...
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Human T-Cell Isolation
(1)
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May 12, 2016
The kit provided by STEMCELL allows for facilitated isolation of human lymphocytes from peripheral blood mononuclear cells. It involves a ...
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Great Cytotoxic T-Cell Marker
(1)
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Tonbo Biosciences
May 12, 2016
Flow cytometry was used to probe epithelial dynamics and the immune infiltrates in human prostate tissues. Epithelia was investigated with ...
Read Review
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Western Blot
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Adipose
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Adipocyte
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