Clean Monocyte Isolation

Washington University in Saint Louis
Surgery
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Mitenyi Biotec

Product Name:

Monocyte Isolation Kit

Catalog Number:

130-100-629

Monocytes were isolated from a spleen from a wildtype B6 mouse. Single cells were isolated and monocytes were isolated per manufacturer's protocol.

Experimental Design and Results Summary

Application

Monocytes isolation for adoptive transfer

Starting Material

Wildtype B6 mouse spleen

Protocol Overview

Monocytes were isolated from C57BL/6 spleen cells by using the Monocyte Isolation Kit (BM), mouse with LS Column

Tips

Timing is important. Work as fast as you can.

Results Summary

Was able to isolate Lin-CD11b+Lyc6+CD11c-/F4/80-/I-Ab- monocytes for adoptive transfer

Additional Notes

None

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Summary

The Good

Easy to use. Straightforward.

The Bad

A little pricey per cell isolation

The Bottom Line

Great way to get quick monocytes isolation!

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