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Date Published
Superb anti-Sheep Secondary Antibody
(1)
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Thermo Fisher Scientific
August 05, 2016
This reagent is a highly sensitive secondary antibody. We analyze samples of crude Arabidopsis thaliana (mouse-ear cress) protein extract ...
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Very Good Anti-Mouse CD8 Antibody
(1)
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BD Biosciences
August 05, 2016
The objective of this experiment was to study T cell subsets in mouse spleens. I tested if the anti-CD8 PerCp antibody from BD can be used ...
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Anti-GAPDH (Western Blot)
(1)
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MilliporeSigma
August 05, 2016
Rat tissue panel analyzing the differential expression level of common endogenous loading control genes. EMD Millipore (#CB1001) anti-GAPDH ...
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Biorbyt CWC22 Review
(1)
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Biorbyt
August 04, 2016
Our lab generates a lot of protein knockouts in mouse embryonic stem cells using crispr-cas9 technology. We use Western Blots and subsequent...
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Direct-Zol RNA MiniPrep Kit -- A Reliable, Good-Value Kit For RNA Isolation
(1)
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August 04, 2016
Our lab uses this kit to isolate mRNA from plasmablasts cultured in vitro. We disrupt the cultured cells in TRIzol, use this kit to purify ...
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Phospho-Cdk Antibody With High Background
(1)
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Santa Cruz Biotechnology, Inc.
August 04, 2016
Antibody directed against phospho-Cdk which produces a high background.
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Mouse mAb sc-377229 from Santa Cruz against EGFR
(1)
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Santa Cruz Biotechnology, Inc.
August 04, 2016
The mouse mAb sc-377229 from Santa Cruz was used to detect EGFR in the lysates of human tumor cells. The antibody produced excellent results...
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Good Loading Control Antibody
(1)
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Abcam
August 04, 2016
Beta actin is cytoskeleton protein and is used as a loading control for western blotting. Since it is ubiquitously expressed in all ...
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Effectiveness Of Rabbit Anti-14-3-3 Epsilon To Detect The Protein In Mouse Ovaries By Immunohistochemistry
(1)
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Bio-Rad
August 04, 2016
The 14-3-3 (YWHA) proteins are a highly conserved, ubiquitously expressed family of proteins controlling important cellular processes, with ...
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Anti-LMAN1 (Western Blot)
(1)
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Abcam
August 04, 2016
LMAN1 immunodectection of endogenous HepG2 LMAN1 protein in siRNA transfected cells.
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Staining PBMC With Anti-CD8 Alexa488
(1)
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BioLegend
August 04, 2016
This antibody was used to phenotype T cells in human peripheral blood. It was chosen because it is a commonly used clone, Alexa488 is very ...
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#AF909 Is Good Antibody
(1)
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R&D Systems, a Bio-Techne Brand
August 04, 2016
Aim of this research was search for specific anti-MMP-9 antibody. Company recommended this antibody to be specific. To validate it I ...
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SYBR Green Based Real Time PCR For Gene Expression Studies In Salmonella Enterica
(1)
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Bioline
August 04, 2016
I used SensiFAST Lo-Rox Kit for measuring Salmonella enterica gene expression under bile salt stress. The standard curve analysis using ...
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Anti-MMP2 (Western Blot)
(1)
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MilliporeSigma
August 03, 2016
Western blot of HEK293 cells over expressing human MMP2 cDNA.
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Rabbit anti-CDCP1 antibody #4115 from Cell Signaling
(1)
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Cell Signaling Technology
August 03, 2016
Rabbit antibody #4115 from Cell Signaling is an excellent antibody for detection of CDCP1 in human tumor cells. It recognizes both the full-...
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Great Loading Control Antibody
(1)
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Santa Cruz Biotechnology, Inc.
August 03, 2016
GAPDH is a commonly used loading control for western blotting and qPCR. Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH) is ...
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Good Antibody If You Can Compensate Well
(1)
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BioLegend
August 03, 2016
CD4 is expressed on a subset of T cells in thymus, spleen and peripheral lymphoid organs. Although the expression of CD4 might change during...
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Anti-MMP9 Antibody Detect Real Signal
(1)
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August 03, 2016
Aim of this research was search for specific anti-MMP-9 antibody. Company recommended this antibody to be specific. To validate it I ...
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Total RNA Extraction From Salmonella Enterica Cultures For qPCR Experiments
(1)
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QIAGEN - Sample to Insight
August 03, 2016
I used Qiagen's RNeasy Midikit for extraction of total RNA from the Salmonella enterica cultures grown in LB medium with different levels of...
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Application Of The 3,3'-Diaminobenzidine (DAB) Substrate In ABC Immunohistochemical Staining
(1)
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August 03, 2016
The 14-3-3 (YWHA) proteins are a highly conserved, ubiquitously expressed family of proteins controlling important cellular processes, with ...
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Staining Mouse RBC With Anti-TER-119 BV421
(1)
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BioLegend
August 03, 2016
This antibody was used as an RBC marker in a hematopoietic lineage staining panel. It was chosen because it is a commonly used clone, BV421 ...
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Good Antibody For MSCs Identification
(1)
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Miltenyi Biotec
August 03, 2016
This antibody is good for MSCs phenotype. MSCs should be positive for CD105 and this antibody works well for this purpose.
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Cdk Antibody With High Unspecificity
(1)
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Santa Cruz Biotechnology, Inc.
August 03, 2016
Target: Arabidopsis CDKB1;1. Did not work with two different goat anti-rabbit secondary antibodies in various concentrations from Thermo or ...
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Anti-Munc13 Is Good Antibody
(1)
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Synaptic Systems GmbH
August 02, 2016
Aim of this research was to detect intensity of Munc13 protein in active zones of Substance P and Lectin positive neurons. This anti-Munc13 ...
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Quantification Of DNA Or RNA Samples Intended To Be Used In Next Generation Sequencing Experiments
(1)
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Thermo Fisher Scientific
August 02, 2016
DNA or RNA samples which are intended to be used in the next generation sequencing experiments were quantified using Qubit based ...
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Good Antibody For Western Blotting
(1)
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Cell Signaling Technology
August 02, 2016
Cyclin D1 is a marker of G0-G1 cell cylce arrest. We treated our cells with a known drug and observed its effect on cell cycle.
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Rabbit polyclonal antibody AP19833a against the N-terminus of EGFR from Abgent
(1)
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August 02, 2016
Rabbit polyclonal antibody AP19833a against the N-terminus of EGFR from Abgent was probed for western blot detection of the recombinant N-...
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Excellent Kit For The Extraction Of DNA From Gels
(1)
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QIAGEN - Sample to Insight
August 02, 2016
This kit was used for isolating PCR fragments from agarose gels. This can also used for isolating DNA from poly acrylamide gels.
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Cdk Antibody With High Unspecificity
(1)
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Santa Cruz Biotechnology, Inc.
August 02, 2016
Target: Arabidopsis CDKA;1. Did not work with two different goat anti-rabbit secondary antibodies in various concentrations from Thermo or ...
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Staining Mouse RBC With Anti-TER-119 FITC
(1)
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BioLegend
August 02, 2016
This antibody was used as a RBC marker in a hematopoietic lineage panel. It was chosen because it is a commonly used clone, spillover from ...
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Antibody
(1)
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Tonbo Biosciences
August 02, 2016
The aim of my research is to study T cells in order to better understand the human immune system. This experiment was to use a new antibody.
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Good TCRb, I Like It Better Than CD3 Staining
(1)
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BioLegend
August 02, 2016
Good Ab for flow. We study inflammation during acute Mtb infection, and as a result, our timepoints look at primary cells from lungs from ...
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Removal Of Genomic DNA Contamination During Total RNA Extraction From Salmonella Enterica Samples
(1)
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QIAGEN - Sample to Insight
August 01, 2016
During total RNA extraction from the Salmonella enterica (bacterial samples in general), small percentage of genomic DNA is also co-eluted. ...
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Single Cell Isolation From Various Tissues
(1)
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Miltenyi Biotec
August 01, 2016
Our lab focused on the clonal heterogeneity in various tissues. As part of our project, need to isolate single cells from various tissues (...
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HA Tag Antibody Is Excellent For ICC
(1)
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BioLegend
August 01, 2016
Goal of this research is finding specific anti-HA antibody. After transfection of HA-CAS to PC12 cells, specific anti-HA signal was detected...
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A Decent Antibody
(1)
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Cell Signaling Technology
August 01, 2016
PCNA is a proliferating Cell marker. We checked PCNA expression in several cells to assess their proliferation abilities.
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Staining Mouse WBC With Anti-CD4 PerCP/Cy5.5
(1)
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BioLegend
August 01, 2016
This antibody was used to phenotype CD4+ T cells in peripheral mouse blood. It was chosen because it is a commonly used clone, PerCP/Cy5.5 ...
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Bright Staining Of CD31 On Human PBMC By Flow Cytometry Using An Anti-Human CD31 Monoclonal Antibody (Clone WM59)
(1)
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BioLegend
August 01, 2016
Our lab routinely uses flow cytometry to measure the expression of various molecules on human peripheral blood mononuclear cells under ...
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Good Anti-Mouse CD3 Antibody
(1)
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BioLegend
August 01, 2016
We used this antibody to determine the percentage of CD3 positive T cells in the spleen of Balb/C mouse. Single cell suspensions were ...
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Cdk Antibody With Low Specificity
(1)
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Santa Cruz Biotechnology, Inc.
August 01, 2016
target: S. cerevisiae Cyclin-dependent kinase 28p. Did not work with two different goat anti-rabbit secondary antibodies in various ...
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Anti-Tubulin mouse mAb #627901 from Biolegend
(1)
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BioLegend
August 01, 2016
Anti-Tubulin mouse mAb #627901 from Biolegend is an excellent antibody to verify protein loading in western blot applications.
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Extraction Of Good Quality Total RNA From Salmonella Enterica For qPCR And RNA-Seq Experiments
(1)
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QIAGEN - Sample to Insight
July 29, 2016
I used Qiagen's RNAprotect Bacteria Reagent to stabilise RNA prior to extraction step so that whatever the gene expression measured after ...
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Staining Mouse WBC With Anti-F4/80 APC
(1)
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BioLegend
July 29, 2016
This antibody was used to stain macrophages in mouse peripheral blood. It was chosen because it is a commonly used clone, a bright ...
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Decent Antibody
(1)
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Santa Cruz Biotechnology, Inc.
July 29, 2016
p21 is a kinase inhibitor which contributes to the regulation of cell division, mediation of negative growth signals, differentiation and ...
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Does Not Work With Mouse Kidney Samples
(1)
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Cell Signaling Technology
July 29, 2016
We are studying the cause of injury which occurs during cold ischemia when kidneys are stored in cold before transplantation. Histology ...
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AbCam#81887 Is Good Antibody
(1)
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Abcam
July 29, 2016
Aim of this research was detection of synaptotagmin intensity in CGRP positive synaptic terminals. This CGRP antibody was recommended by ...
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Rat Anti-Mouse CD31 (Clone MEC13.3) Performs Well In Flow Cytometry
(1)
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BioLegend
July 29, 2016
The general aim of our research is the study of lymphocyte biology and how this varies with health and disease. The specific goals of the ...
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Anti-beta Actin (Western Blot)
(1)
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July 29, 2016
Rat tissue panel analyzing the differential expression level of common endogenous loading control genes. Sigma Aldrich (#A1978) anti-beta ...
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A Perfect Plasmid Isolation Kit
(1)
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QIAGEN - Sample to Insight
July 29, 2016
A kit comess with all the reagents and a column to obtain quality amount of plasmid DNA.
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Anti-MBP Purification Tag Antibody
(1)
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New England Biolabs
July 29, 2016
MBP affinity purification tag can be easily detected using this antibody and therefore used as loading or interaction control in various ...
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Western Blot
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Adipose
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Adipocyte
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