Staining AML Cell Line With Anti-CD45 BV421

August 18, 2016

Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Anti-human CD45 BV421

Catalog Number:

304032

This antibody was used in combination with anti-CD33 PE as a marker for AML cells in a xenograft mouse model. It was chosen because it is a commonly used clone for CD33, BV421 is bright and works well with PE, and Biolegend had the best price.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes from AML model mouse

Primary Incubation

5uL per test in 100uL total staining volume for 20 minutes at room temperature

Blocking Agent

1:50 dilution of anti-mouse and anti-human FcBlock and 2% FBS.

Secondary Incubation

n/a

Tertiary Incubation

n/a

Detection

n/a

Results Summary

CD45 staining was as expected, with nearly all of the splenocytes recovered from AML-bearing mice being positive in the BV421 channel.

Additional Notes

None

Related Categories

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Summary

The Good

Works well. Low cost

The Bad

n/a

The Bottom Line

I will continue to use this antibody.

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