Staining PBMC With Anti-CD4 BV510

Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

Anti-human CD4 BV510

Catalog Number:

562970

This antibody was used in a T cell phenotyping panel. It was selected because it is a commonly used clone, and BV510 was a good fluorochrome to label CD4 cells with in this panel.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC

Primary Incubation

5uL per test in 100uL total staining volume for 20 minutes at room temperature

Blocking Agent

2% FBS

Secondary Incubation

n/a

Tertiary Incubation

n/a

Detection

n/a

Results Summary

CD4 staining was as expected and the positive cell signal was fully resolved from negative cells.

Additional Notes

None

Related Categories

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Summary

The Good

Works well.

The Bad

High cost

The Bottom Line

I will definitely continue to use this antibody in this staining panel.

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