Biolegend
Anti-human CD268-AF647 (BAFF-R)
316914
Info: View Product View Product Specs
Browse Similar Products: CD268 Flow Cytometry Antibodies
Identification of BAFF-R positiv cells within a B-Cell population.
Flow Cytometry
Human whole blood
1. 80 µl EDTA blood plus 20 µl antibody cocktail (in FACS Buffer), incubate for 30 min at RT.
None
2. Lysis of erythrocytes by adding 1 ml of Lysis Buffer, incubate for 10 min at RT. 3. Wash two times with PBS; centrifuge at 400 x g for 5 min, trash supernatant. 4. Resuspend cells in 200-300 µl FACS Buffer, FACS analysis.
N/A
This antibody was titrated before use and can be used highly diluted at a final dilution of 0.1µl AB per 100µl volume.Very bright and easy to detect.
Gating Strategy: Lymphocytes > single cells > CD19+ CD20+ Bcells > CD268+
Can be diluted very high
Nothing
Very bright and easy to detect.