A Nice Antibody For Detection Of p21

Department of Microbiology and Immunology
University of Illinois, Chicago
PostDoctoral Research Fellow

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Company:

Cell signaling

Product Name:

p21 Waf1/Cip1 (12D1) Rabbit mAb

Catalog Number:

2947

Tumor suppressor genes/proteins are important regulators of cell cycle. They hamper the cell cycle if there is any mistake or error during the cell division. The tumor suppressor protein p21 Waf1/Cip1 acts as an inhibitor of cell cycle progression. These proteins are important to prevent tumor development. We used siRNA against our gene of interest and determined its effect on levels of p21.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Whole cell lysates treated with different toxic agents

Primary Incubation

Diluted 1:500 in blocking solution, incubated for overnight at 4 degrees

Blocking Agent

5% non BSA in TBST

Secondary Incubation

Anti-rabbit secondary antibody

Tertiary Incubation

None

Detection

ECl

Results Summary

This antibody works well in western and is very sensitive in its reactivity.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

No non specific bands

The Bad

Nothing

The Bottom Line

It is of the best antibodies from cell signaling.

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