Abnova
CAMKK2 monoclonal antibody (M02)
H00010645-M02
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The CAMKK2 siRNAs were treated to cells for 72 hours and efficacy and effectiveness of siRNA in CAMKK2 protein knockdown needed to be determined. Western blotting of CAMKK2 using the antibody (H00010645-M02) confirmed the efficient knockdown.
Western Blot
CFBE41o- cells
Primary antibody dilution buffer (TBST with 5% BSA) with gentle agitation overnight at 4°C.
TBST with 5% w/v nonfat dry milk
Incubated the membrane with the Goat Anti-Mouse IgG, H & L Chain Specific Peroxidase Conjugate (Cat No: 401253 Merckmillipore) 1:10000 diluted in 10 ml of blocking buffer with gentle agitation for 1 hr at room temperature.
None
Incubated the membrane with appropriate amount of Luminata Crescendo Western HRP substrate (WBLUR0100 Merckmillipore) for 5 min at room temperature. Drained the membrane of excess developing solution (without letting it to dry) wrapped in transparent plastic sheet and exposed the x-ray film for 1 min.
Antibody works well for the Western blotting of CAMKK2.
Sensitive reagent for western blotting.
Good reagent.