Bio-Rad
TGN46 Antibody
AHP500GT
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Browse Similar Products: TGN46 Immunofluorescence Antibodies
TGN46 is a trans-Golgi localized protein and it can be used to access any protein that may be localized to trans-Golgi by estimating the co-localization with TGN46.
Immunofluorescence
Hela cells
Primary antibody diluted 1:500 in 1% BSA- PBS and incubated the coverslips with cells for 1 hour at room temperature
5% BSA prepared in PBS for 1 hour at room temperature.
Donkey anti-sheep IgG (H+L) Secondary Antibody, Alexa Fluor® 568 conjugate(A-21099 thermo fisher) 1:400 diluted in 1% BSA- PBS incubated for 1 hour at room temperature
None
Coverslips were inverted onto a glass slide with a drop of mounting media and Imaged using Zeiss LSM 700 confocal fluorescence microscope
The TGN46 antibody (AHP500GT, Bio-Rad) stains TGN46 with very low background or unspecific labeling.
No unspecific labeling.
Good TGN46 antibody