Abbexa
GPRC6A Antibody
abx015319
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The aim of this piece of research was to identify whether the GPRC6A receptor is present or not in a human cell line in which it had not been confirmed previously. We used the GPRC6A receptor from Abbexa for a quick and visual answer to this question. We selected the Abbexa product in particular as it also stated it can be applied in western blotting, immunofluorescence and ELISA experiments and this was appealing to us as we wanted to do multiple verifications of the presence of the receptor in our cell line. Furthermore we will use this product to distinguish whether we are able to successfully knock-down the GPRC6A protein expression in future experiments.
Immunofluorescence
Human cell line
GPRC6A 1:100 dilution, overnight at 4 degrees Celsius with shaking
20% goat serum in PBS
Goat anti-rabbit Alexa Fluor 488® conjugated; 1:1000 for 30 minutes at room temperature with shaking and protected from light
N/A
Fluorescent microscope
Very happy with results
None
Dilution at 1:100 perfect on first go
Nothing to report!
May have struck lucky but worked perfectly first time with immunofluorescence, will try next with western blotting.