Novus Biologicals
GRASP55 Antibody
NBP1-89747
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GRASP55 is important for assembly and membrane stacking of the Golgi cisternae. It can be used as a Golgi marker.
Immunofluorescence
HeLa cells
Primary antibody diluted 1:200 in 1% BSA- PBS and incubated for 1 hour at room temperature.
5% BSA prepared in PBS
Coverslips were incubated for 1 hour at room temperature (in a dark chamber) with goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate 1:400 diluted in 1% BSA- PBS.
None
Coverslips were inverted onto a glass slide with a drop of mounting media and Imaged using Zeiss LSM 700 confocal fluorescence microscope.
The antibody marks the Golgi as expected.
It works well as described
Good antibody to for detecting GRASP55 immunofluorescence.