Abcam Inc.
Anti-beta III Tubulin
ab78078
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This antibody was used in a study to quantify the extent of neurite outgrowth after NGF stimulation in PC12 cells (pheochromocytoma cell line derived from the rat adrenal medulla). PC12 cells were stimulated with 100 ng/mL NGF and 96 hours later neurite outgrowth was quantified by performing immunocytochemistry of fixed cells stained for beta III tubulin followed by quantification of immunofluorescence pixel density using Image J software.
Immunofluorescence
PC12 cells grown in 24-well tissue culture dish were stimulated and incubated in 100 ng/mL NGF for 4 days in order to allow neurite formation. Cells were fixed with 4% PFA fore proceeding with the immunocytochemistry protocol.
A dilution of 1:1000 of anti-beta III Tubulin in 2% DGS was incubated at room temperature for 2 hours.
10% Donor Goat Serum (DGS)
A dilution of 1:250 of anti-Mouse-594 (Alexa series) in 2% DGS was incubated at room temperature for 1 hour.
N/A
594nm excitation on an inverted microscope.
The anti-beta III Tubulin gave a very strong signal even at a low dilution (1:1000). Beta III Tubulin stained the neuron cell body and neurites allowing the efficient quantification of neurite outgrowth using Image J software.
Anti-beta III tubulin (ab78078) is capable of detecting the rat eptiope.
The antibody gave a very strong signal at a very low dilution.
None to report.
The antibody worked great, I would highly recommend to researches looking for a high quality beta III Tubulin antibody.