BD Biosciences
Purified, Mouse, Anti-Human FAK (pY397)
611722
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Western blot assays to validate protein array data in ovarian cancer cell lines.
Western Blot
Ovarian cancer cell line panel lysates
1:1000 in 5% milk in TBS-T, overnight at 4 degrees C, on a rocker
5% milk in TBS-T
1:5000, anti-Ms in 5% milk in TBS-T, 30 minutes at room temp, on a rocker
None
HRP Chemiluminescence
Blot reveals two bands, one that corresponds with the FAK monomer size (125 kDa) and one that corresponds with the FAK dimer (~250 kDa).
Can visualize both the monomer and dimer using this antibody
Good antibody, clear bands at the correct size.