Abcam
Anti-IRE1 antibody
ab37073
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Browse Similar Products: IRE1 Western Blot Antibodies
IRE1alpha is a key protein that is phosphorylated and activated during ER stress, initiating the unfolded protein response. Determining the activation state of IRE1alpha due to a variety of stress conditions is helpful in assessing the severity of ER stress.
Western Blot
Rat hepatoma H4IIE liver cells
1:500 in 1:3 odyssey block:1X TBST (0.1% Tween) shaking, overnight at 4 degrees C
1:3 odyssey block:1X TBS at room temperature for 1 hr.
1:15000 Goat anti-rabbit IRDye in 1:3 odyssey block:1X TBST (0.1% Tween) and 0.2% SDS for one hour at room temperature in the dark
N/A
Licor Odyssey
Total IRE1alpha is pretty clear at 125kDa but there is a ton of non-specific binding and two prominent bands near 40kDa that show up so strong they mask the signal from IRE1. Have to adjust the brightness and contrast of the image to see IRE1alpha.
None
Antibody works.
A lot of background.
This is the first IRE1 antibody that has worked in our cell line. Would recommend for others if no other IRE1 antibody from other companies have worked.