Excellent Purified Mouse Anti-beta Catenin Antibody

August 22, 2016

BlueskyReddit
Pathology
University of Kansas Medical Center
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BD Biosciences

Product Name:

Purified mouse anti-Beta Catenin

Catalog Number:

610154

Western blot assays to validate protein array data in ovarian cancer cell lines.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Ovarian cancer cell line lysates

Primary Incubation

1:250, O/N, 4 degrees C

Blocking Agent

5% milk in TBS-T

Secondary Incubation

1:5000, anti-Ms in 5% milk in TBS-T, 30 minutes at room temp, on a rocker

Tertiary Incubation

None

Detection

HRP Chemiluminescence

Results Summary

Blot revealed single, crisp bands just below the 100 kDa marker. The predicted molecular weight of beta-catenin is 92 kDa so the bands appear correct.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Best anti-beta-catenin antibody I've ever used (I tested 4 or 5 before choosing this one)

The Bad

Very expensive, with no option for a test size.

The Bottom Line

Good antibody, produces a single band. Expensive, but worth it if you'll be evaluating beta-catenin a lot.

Share your experience with other scientists. Write a Review! »

Join the discussion