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Antibodies
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DuoSet CTLA-4 ELISA
(1)
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R&D Systems, a Bio-Techne Brand
September 14, 2018
Our laboratory focuses on effects of aryl hydrocarbon receptor (AhR) activation in dendritic cells. I used the DuoSet CTLA-4 ELISA kit so ...
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Antibody to Detect SLUG in Human Carcinoma Cells
(1)
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Santa Cruz Biotechnology, Inc.
September 14, 2018
SLUG, the protein product of the SNAI2 gene, is a member of the Snail family of zinc-finger transcription factors. It is involved in the ...
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RNA Extraction Kit for Samples with High Lipid Content
(1)
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QIAGEN - Sample to Insight
September 14, 2018
We use this kit when extracting RNA from tissues or cells with high content of lipids, such as brain or fat tissue. We have use with ...
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Cell Count and Viability Using the LUNA-FL
(1)
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Logos Biosystems
September 14, 2018
We use LUNA-FL for counting and measuring cell viabilities prior submitting the cells to single cell RNA-seq analysis. LUNA-FL was chosen ...
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Human Anti-CD20-APC-Vio770 Antibody
(1)
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Miltenyi Biotec
September 14, 2018
This anti-CD20 antibody was tested in order to establish a panel to describe and distinguish peripheral blood lymphocytes (from human PBMCs)...
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Removal of Nucleic Acids in Protein Extraction
(1)
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September 14, 2018
Separation of protein on chromatography columns from proteins extracted from gram negative bacteria treated with 1X BugBuster lysis reagent ...
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Competent E. coli for Cloning Constructs with High Efficiency
(1)
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New England Biolabs
September 13, 2018
Routing cloning plasmid constructs of size less than 8 Kb constructed by restriction cloning were transformed into NEB 5 alpha high ...
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Kit for RNA Extraction
(1)
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QIAGEN - Sample to Insight
September 13, 2018
With this kit we extracted RNA from murine myoblasts cultured in vitro in order to see the expression of several genes. In the picture we ...
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Stains Clear Population of Cxcr3+ T Cells, But Not Particularly Bright
(1)
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BioLegend
September 13, 2018
Our goal was to analyze the response different cell types in mouse lungs. Mouse lungs were digested with collagenase, strained, and stained...
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Use of Anti-DISC1 Antibody in ICC
(1)
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Abcam
September 13, 2018
Our lab aimed to determine the cellular processes involved in T cell activation, and we hypothesized that DISC1 plays a crucial role in ...
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Good Option to Stain LAG-3 in Mouse
(1)
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Thermo Fisher Scientific
September 13, 2018
B cells have been shown to play a major role in pathogenesis of autoimmune diseases through presentation of antigen to T cells, production ...
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Great Antibody for Mouse CD5
(1)
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BioLegend
September 13, 2018
Our lab is interested in studying the T cells that are involved in the clearance of mouse influenza virus. CD5 is expressed on T cells and ...
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Anti-Human CD19 BV605 Antibody
(1)
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BioLegend
September 13, 2018
This anti-CD19 antibody was tested in order to establish a panel to describe and distinguish peripheral blood lymphocytes. We are ...
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Clear ICOS Labeling, But Shows a Gradient
(1)
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BioLegend
September 12, 2018
Our goal analyze the response different cell types in mouse lungs after pulmonary bacteria infection. Mouse lungs were digested with ...
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Electrocompetent E. coli for Transforming Large Constructs
(1)
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New England Biolabs
September 12, 2018
I have constructed large plasmid having gene interest to clone into recipient bacteria by Gibson assembly method. The constructed plasmid ...
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Good Option for CD43 Staining in Mouse
(1)
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BD Biosciences
September 12, 2018
My research aims at the elucidation of the roles of B cells and plasma cells in health and disease. In this experiment, I aimed at analyzing...
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Good Antibody for Mouse Ly6c
(1)
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BioLegend
September 12, 2018
Our lab is interested in studying the T cells that are involved in the clearance of mouse influenza virus. Ly6c is known to be expressed on...
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Western Blot of HeLa Cell Lysates Using H4 Specific Antibody
(1)
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Abcam
September 12, 2018
Our goal was to determine the levels of H4K16 acetylation in control vs protein X knockdown in whole cell lysates of HeLa cells. Pan-H4 ...
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Good Antibody to Detect Human Twist Protein
(1)
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Santa Cruz Biotechnology, Inc.
September 12, 2018
Twist (also known as twist-related protein 1, or TWIST1) belongs to the group of basic helix-loop-helix proteins regulating cell ...
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Anti-TRPC6 Tried in Human Tissue Sample
(1)
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BosterBio
September 12, 2018
We are comparing the expression of the TRPC6 protein in psychological symptoms characterized in a human tissue lysate. This Anti-TRPC6 ...
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Kit to Demonstrate Hypoxia In Vivo
(1)
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September 11, 2018
High levels of hypoxia in vivo are associated with the induction of tumor angiogenesis, which in turn, is crucial for primary tumor growth ...
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Good Anti-Histone H2A.Z Antibody
(1)
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Abcam
September 11, 2018
Our goal was to study the stability of H2A in the nucleosomes. For that, we immunoprecipitated Flag-H2A nucleosomes from yeast cell lysates ...
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Anti-TRPC5 Tried in Human Tissue Sample
(1)
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BosterBio
September 11, 2018
We are comparing the expression of the TRPC5 protein in psychological symptoms characterized in a human tissue lysate. This Anti-TRPC5 ...
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Extraction Kit for Large DNA Inserts After Digestion
(1)
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QIAGEN - Sample to Insight
September 11, 2018
QIAEX II Gel extraction kit was used to gel excise and extract DNA inserts of sizes ranging from 15 to 20 kb from 0.7 % agarose gel. Using ...
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Good CD44+ Mouse Antibody
(1)
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BioLegend
September 11, 2018
Our lab is interested in studying the T cells that are involved in the clearance of mouse influenza virus. In order to study their effector...
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Excellent Histone H3 Antibody for Western Blot
(1)
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Abcam
September 11, 2018
Our goal was to examine the global levels of H3 in lysates of HeLa and 293T cells by western blot. We lysed both the cell lines in NETN ...
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Antibody Labeling of Blood Resident Lymphocytes In Vivo
(1)
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BioLegend
September 11, 2018
Our goal was to distinguish lymphocytes that reside in the vasculature vs those that reside in the tissue in the lungs. We injected 3ug (...
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OK Option to Stain Mouse LAG-3
(1)
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Thermo Fisher Scientific
September 10, 2018
B cells have been shown to play a major role in the pathogenesis of autoimmune diseases through presentation of antigen to T cells, ...
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Collagenase for Mammary Gland Primary Cell Culture
(1)
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September 10, 2018
I have used collagenase A from Sigma Aldrich at 2.5 mg/ml concentration to treat mammary gland tissue to get single cell suspensions in DPBS...
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Very Helpful HB-EGF ELISA Kit for Human Samples
(1)
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R&D Systems, a Bio-Techne Brand
September 10, 2018
Background: Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a rational target for ovarian cancer therapy. The aim of ...
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Excellent Antibody to Detect Human MMP-9
(1)
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Santa Cruz Biotechnology, Inc.
September 10, 2018
Matrix metalloproteinases (MMPs) are important enzymes involved in many physiological processes, including matrix remodeling and cancer ...
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Multi-block PCR System with Touchscreen Issues
(1)
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Thermo Fisher Scientific
September 10, 2018
Our lab brought Proflex 3x32 well in 2015 primarily for genotyping assays. Unfortunately for us the machine worked well until its warranty ...
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Clear Population, but PE is Unnecessarily Bright
(1)
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BioLegend
September 10, 2018
Our goal was to analyze the response different cell types in mouse lungs after pulmonary bacteria infection. Mouse lungs were digested with...
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Great KLRG1 Mouse Antibody
(1)
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BioLegend
September 10, 2018
Our lab is interested in studying the T cells that are involved in the clearance of mouse influenza virus. Specifically we are looking at ...
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Good Antibody for T Cell Activation, Paired with APC-CD44
(1)
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BioLegend
September 07, 2018
T cell activation leads to down regulation of CD62L and up regulation of CD44. We used PE-CD62L and APC-CD44 to detect CD8 T cell activation...
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Total RNA Extraction Kit for Gram Negative Bacteria
(1)
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New England Biolabs
September 07, 2018
I have recently used New England Biolabs Total RNA Miniprep kit to extract RNA from Gram negative bacterial cell pellets. Using 10^10 cells ...
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CD8 Antibody That Works Well
(1)
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BioLegend
September 07, 2018
Our lab studies the roll of T cells in the clearance of influenza virus and memory functions. To do any analysis of these T cells, we need ...
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DNase I for Removal of Contaminating DNA
(1)
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September 07, 2018
I have used DNase I from Sigma Aldrich to remove contaminating genomic DNA during total RNA extraction from the gram negative bacterial ...
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Anti-CD45 for Labeling of Mouse Lung Immune Cells
(1)
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BD Biosciences
September 07, 2018
We used this antibody to monitor various cell types in the mouse lungs during pulmonary infection. Mouse lungs were digested with ...
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Good Kit for Cloning Screening
(1)
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QIAGEN - Sample to Insight
September 07, 2018
We use this kit for extracting small amounts of plasmids from different Escherichia coli colonies to confirm the presence of a clone inside ...
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Usable Anti-Mouse IgG1
(1)
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Thermo Fisher Scientific
September 07, 2018
Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against ...
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Good Antibody to Detect Human TGF Beta 1
(1)
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Bio X Cell
September 06, 2018
TGF-ß1 is an important growth factor ubiquitously expressed in normal and cancer cells. It is produced as a Small Latent Complex (SLC), ...
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Good CD86 Antibody
(1)
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BD Biosciences
September 06, 2018
In this project the phenotyping of immune cells in murine skin and lymph node was investigated. The immune cells were attracted and ...
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Great GFP Antibody for IHC in the Drosophila Eye
(1)
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GeneTex
September 06, 2018
We express split-GFP 1-10 beta sheets in fly eye and used this antibody to detect the signals of GFP. IHC results are clear with little ...
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Extraction of High Amounts of Plasmid
(1)
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QIAGEN - Sample to Insight
September 06, 2018
We use this kit for extraction of high amounts of plasmid from Escherichia coli. Since we use this plamids in non viral gene therapy and ...
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Bright Antibody That Works Well for CD3 Cells
(1)
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BioLegend
September 06, 2018
Our lab focuses on the study of T cells and all their different subsets. In order to do any analysis, we need a reliable CD3 marker so we ...
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Anti-Mouse CD49a for Labeling iNKT Cells
(1)
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BD Biosciences
September 06, 2018
We used this antibody to label a subset of iNKT cells from mouse lung tissue.
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Intracellular IgM Staining
(1)
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BioLegend
September 06, 2018
Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against ...
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Good Antibody to Stain Intracellular IgG3
(1)
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BD Biosciences
September 05, 2018
Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against ...
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All-Purpose Workhorse in the Lab for Quantification
(1)
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September 05, 2018
Among many alternatives available, the NanoDrop 2000 is an all-purpose workhorse in the lab. It is a fantastic device to measure DNA and RNA...
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ELISA Kits
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Kits
Labels / Dyes / Stains
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Western Blot
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Immunoprecipitation
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Cell Culture
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qPCR
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RT-PCR
cDNA Synthesis
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Multiplex
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RNA-Seq
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Concentration
HPLC
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Chromatography
Mass Spectrometry
Labeling
Microarray
Mutagenesis
FISH
CRISPR
TUNEL Assay
In Vivo Imaging
EMSA
Cell Fractionation
Immunoassay
In Vitro Transcription
ChIP-Seq
Northern Blot
3D Imaging
RNAi
ELISPOT
PCR Cloning
Particle Analysis
Southern Blot
Protein Modification
DNA Analysis
Expression
Cell-Based Assay
Gene Editing
In Vivo Testing
Protein Labeling
Other
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Human
Mouse
Rat
Non-Human Primate
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Arabidopsis
Yeast/Fungi
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Virus
Other Invertebrate
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Drosophila
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Rhesus
Tissue
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Adipose
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In Vivo
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Peripheral Blood
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Thyroid Gland
Tonsil
Trachea
Umbilical Cord
Umbilical Vein
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Vein
Cell Type
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Adipocyte
Astrocyte
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CD8+ T Cell
Competent Cells
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Endothelial Cell
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Epithelial Cell
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HUVEC
Induced Pluripotent Stem Cell
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Macrophage
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Microglia
Microvascular Endothelial Cell
Monocyte
Myoblasts
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Neuron
Neutrophil
NK Cell
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Oligodendrocytes
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PBMC
Platelets
Progenitor Cell
Red Blood Cell
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Smooth Muscle Cell
Splenocyte
Stem Cell
Stromal Cell
Synoviocyte
T Cell
Thymocytes
Tumor Cells
Cell Line
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HeLa
PC3 (PC-3)
HEK293
HEK293T (HEK-293T)
CHO
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THP-1
U2OS
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HL60 (HL-60)
A375 (A-375)
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MCF-7
A431
MDA-MB-231
A549
N2a
Daudi
HCT-116
Raji
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