Ly-6G Antibody for Flow Cytometry

October 17, 2018

Advanced Diagnostics
University Health Network
Postdoctoral Fellow

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Company:

Biolegend

Product Name:

Alexa Fluor® 700 anti-mouse Ly-6G Antibody

Catalog Number:

127621

I study the immune responses within the heart during different forms of heart disease. This Alexa 700 anti-mouse Ly-6G antibody was used to identify neutrophilic infiltrates by flow cytometry in the murine heart during myocarditis.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine cardiac tissue

Primary Incubation

15 minutes

Blocking Agent

anti-CD16/CD32 antibody

Secondary Incubation

30 minutes

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

Alexa 700anti-mouse Ly-6G combined with Side Scatter analysis identifies neutrophils by flow cytometry.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

It works well when combined with Side Scatter

The Bad

Alexa 700 is not the brightest fluorochrome to label Ly-6G

The Bottom Line

This antibody performs very well to label neutrophil in different tissues, including lung, spleen, and heart.

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