IncuCyte Zoom Allows for Live Cell Imaging and Massive Data Processing

October 22, 2018

BlueskyReddit
University of Washington
Immunology
Senior Research Fellow

Overall

Performance

Ease-of-Optimization

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Company:

Sartorius

Product Name:

IncuCyte ZOOM Live Cell Imaging System

Catalog Number:

4459

Our lab uses the IncuCyte ZOOM for numerous live cell assays. We have used it for viral titering, cell death assays, gene expression assays, siRNA transfection efficiency, and more. The system allows for Red, Green and Live Cell imaging in one place with a single interface that has access for multiple workstations with the computing housed in the central computer.

Experimental Design and Results Summary

Application

Viral Titering using a Focus Forming Unit (FFU) Assay

Protocol Overview

Vero cells were infected with West Nile Virus Infectious Clone for 20 hours and then fixed and stained for WNV E protein using a FITC-conjugated antibody. Plates (96 well flat bottom) were imaged on the IncuCyte ZOOM at 4X for whole-well images. Image shows before and after image analysis where the green is the stained virus and the purple is a mask of counted foci.

Tips

Noting the plate brand and catalog number to input into the system helps the program correct for optical density of the plate. Also, ensure that the liquid in the plate contains no bubbles or condensation on the lid to greatly improve image quality.

Features Summary

Simultaneous imaging of phase and green fluorescence. Images up to 6 x 96 well plates from a single setup (start and walk away). Image analysis has a training feature that allows you to adjust the parameters to meet your needs.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Excellent automation for subjective visual assays, greatly improves viral titering speed and allows multiple timepoints from a single continuous plate

The Bad

Only red and green fluorescence. More scanning heats the machine and therefore the incubator which requires cooling time, potentially spacing out timepoint scans

The Bottom Line

Excellent in-incubator live cell plate imager. Highly recommended.

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