Good Histone H3 Antibody for Western Blots

October 11, 2018

School of Graduate Studies
SUNY Downstate Medical Center
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Catalog Number:

ab1791

Our aim was to determine changes in methylation pattern of H3K9me3 by changing Setdb1 expression in PC3 cells. For this study, H3 was used as a control to determine changes in H3K9me3 with respect to total H3 loaded.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human cell lines PC3 lysate

Primary Incubation

Overnight, 4 degree C, 1:10,000

Blocking Agent

5% milk

Secondary Incubation

Goat anti rabbit

Tertiary Incubation

N/A

Detection

Hrp-dab reaction

Results Summary

H3K9me3 was expressed in cell lines, however, changes in methylation pattern were not observed by changing the expression of Setdb1. H3 was used as a control to determine changes in H3K9me3 expression.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Highly specific, no cross reactivity and concentrated

The Bad

None

The Bottom Line

Good control antibody.

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