B Cell Staining among Human PBMC

Tisch Cancer Institute
Icahn School of Medicine at Mount Sinai
Post-Doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

PerCP-Cy™5.5 Mouse Anti-Human CD19

Catalog Number:

561295

Good antibody to identify B cells among human PBMC

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

LN2-stored human PBMC

Primary Incubation

20 min, RT

Blocking Agent

Housemade blocking buffer containing normal sera

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

LSR Fortessa

Results Summary

This antibody was used to identify B cells among primary human PBMC. Although PerCp-Cy5.5 is generally not the brightest color, this antibody gives a good signal-to-noise ratio for easy identification.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Good signal-to-noise ratio

The Bad

Not the brightest signal

The Bottom Line

Easy-to-use antibody for detection of B cells

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